Is Haemophilus influenzae better satellite for Enterococcus faecalis?
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Tarih
2013
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Russian Open Medical Journal
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Background - Haemophilus influenzae can grow on blood agar media with Staphylococcus aureus which can provide factor V as it is called "Satellite phenomenon". Objectives - In this study we tested and compared three different beta-haemolytic genus including three Staphylococcus aureus, three coagulase-negative staphylococci, and two Enterococcus faecalis strains in order to determine an alternative microorganism to be used for satellite test to idenify H. influenzae conventionally. Materials and Methods - We used suspensions of H. influenzae in two different tribudities as 0.5 and 4 McFarland for each strain. Five totally-blinded reviewers examined the test results and scored both the colony sizes of H. influenzae and the diameter of the growth-zone. The sum of the scores for the colony sizes and the growth-zones were determined as "total diagnostic score" (TDS) as being between 0-6 points for each test. Results - A total of 320 test scores were analysed. The mean TDS of E. faecalis group was significantly higher than the other groups (p<0.001). In the S. aureus group, 23 (19.2%) tests had 0 points as TDS; but in enterococci group no isolates had lower scores than 3 points. In enterococci group, the rate of isolates which had 5 or 6 points was 77.5% (62/80); but in S. aureus group no isolate had higher than 4 points. Conclusions - Our study shows that using a beta-haemolytic E. faecalis strain will provide significantly more accurate results and will significantly reduce false-negative results for satellite test instead of S. aureus, which is particularly proposed to be used. © 2012 LLC Science and Innovations, Saratov, Russia.
Açıklama
Anahtar Kelimeler
Enterococcus Faecalis, Haemophilus Influenzae, Satellite Phenomenon, Satellite Test
Kaynak
Russian Open Medical Journal
WoS Q Değeri
N/A
Scopus Q Değeri
Q4
Cilt
2
Sayı
3
