Synthesis of hydrophobic nanoparticles for real-time lysozyme detection using surface plasmon resonance sensor

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dc.authorid0000-0002-1217-5225en_US
dc.authorid0000-0001-5173-1522en_US
dc.authorid0000-0003-3260-1639
dc.authorid0000-0001-7548-5741
dc.contributor.authorSaylan, Yeşeren
dc.contributor.authorYılmaz, Fatma
dc.contributor.authorDerazshamshir, Ali
dc.contributor.authorYılmaz, Erkut
dc.contributor.authorDenizli, Adil
dc.date.accessioned2021-06-23T19:45:31Z
dc.date.available2021-06-23T19:45:31Z
dc.date.issued2017
dc.departmentBAİBÜ, Gerede Meslek Yüksekokulu, Kimya Ve Kimyasal İşleme Teknolojileri Bölümüen_US
dc.description.abstractDiagnostic biomarkers such as proteins and enzymes are generally hard to detect because of the low abundance in biological fluids. To solve this problem, the advantages of surface plasmon resonance (SPR) and nanomaterial technologies have been combined. The SPR sensors are easy to prepare, no requirement of labelling and can be detected in real time. In addition, they have high specificity and sensitivity with low cost. The nanomaterials have also crucial functions such as efficiency improvement, selectivity, and sensitivity of the detection systems. In this report, an SPR-based sensor is developed to detect lysozyme with hydrophobic poly (N-methacryloyl-(L)-phenylalanine) (PMAPA) nanoparticles. The SPR sensor was first characterized by attenuated total reflection-Fourier transform infrared, atomic force microscope, and water contact angle measurements and performed with aqueous lysozyme solutions. Various concentrations of lysozyme solution were used to calculate kinetic and affinity coefficients. The equilibrium and adsorption isotherm models of interactions between lysozyme solutions and SPR sensor were determined and the maximum reflection, association, and dissociation constants were calculated by Langmuir model as 4.87, 0.019nM(-1), and 54nM, respectively. The selectivity studies of SPR sensor were investigated with competitive agents, hemoglobin, and myoglobin. Also, the SPR sensor was used four times in adsorption/desorption/recovery cycles and results showed that, the combination of optical SPR sensor with hydrophobic ionizable PMAPA nanoparticles in one mode enabled the detection of lysozyme molecule with high accuracy, good sensivity, real-time, label-free, and a low-detection limit of 0.66nM from lysozyme solutions. Lysozyme detection in a real sample was performed by using chicken egg white to evaluate interfering molecules present in the medium.en_US
dc.identifier.doi10.1002/jmr.2631
dc.identifier.issn0952-3499
dc.identifier.issn1099-1352
dc.identifier.issue9en_US
dc.identifier.pmid28322473en_US
dc.identifier.scopus2-s2.0-85016634121en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.urihttps://doi.org/10.1002/jmr.2631
dc.identifier.urihttps://hdl.handle.net/20.500.12491/9168
dc.identifier.volume30en_US
dc.identifier.wosWOS:000407478900004en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorYılmaz, Fatma
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofJournal Of Molecular Recognitionen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectHydrophobic Nanoparticleen_US
dc.subjectLysozymeen_US
dc.subjectSensoren_US
dc.subjectSurface Plasmon Resonanceen_US
dc.titleSynthesis of hydrophobic nanoparticles for real-time lysozyme detection using surface plasmon resonance sensoren_US
dc.typeArticleen_US

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