In silico designing and characterization of outer membrane protein K (OmpK) from Vibrio anguillarum and its expression in Nicotiana tabacum for the development of a plant-based vaccine against fish vibriosis

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dc.contributor.authorMalik, Muhammad Suleman
dc.contributor.authorElahi, Iqra
dc.contributor.authorSameeullah, Muhammad
dc.contributor.authorIjaz, Fatima
dc.contributor.authorBatool, Neelam
dc.contributor.authorKhalid, Fatima
dc.contributor.authorGurel, Ekrem
dc.date.accessioned2024-09-25T19:56:22Z
dc.date.available2024-09-25T19:56:22Z
dc.date.issued2024
dc.departmentAbant İzzet Baysal Üniversitesien_US
dc.description.abstractVibriosis is caused by Vibrio anguillarum in various species of aquaculture. A novel, secure, and stable vaccine is needed to eradicate vibriosis. Here, for reverse vaccinology and plant -based expression, the outer membrane protein K (OmpK) of V. anguillarum was chosen due to its conserved nature in all Vibrio species. OmpK, an ideal vaccine candidate against vibriosis, demonstrated immunogenic, non -allergic, and non-toxic behavior by using various bioinformatics tools. Docking showed the interaction of the OmpK model with TLR-5. In comparison to costly platforms, plants can be used as alternative and economic bio-factories to produce vaccine antigens. We expressed OmpK antigen in Nicotiana tabacum using Agrobacterium-mediated transformation. The expression vector was constructed using Gateway (R) cloning. Transgene integration was verified by polymerase chain reaction (PCR), and the copy number via qRT-PCR, which showed two copies of transgenes. Western blotting detected monomeric form of OmpK protein. The total soluble protein (TSP) fraction of OmpK was equivalent to 0.38% as detected by ELISA. Mice and fish were immunized with plant -derived OmpK antigen, which showed a significantly high level of anti-OmpK antibodies. The present study is the first report of OmpK antigen expression in higher plants for the potential use as vaccine in aquaculture against vibriosis, which could provide protection against multiple Vibrio species due to the conserved nature OmpK antigen.en_US
dc.description.sponsorshipHigher Education Commission of Pakistanen_US
dc.description.sponsorshipAcknowledgments We are thankful to the Higher Education Commission of Pakistan for the provision of funds under indigenous scholarship to scholar Muhammad Suleman Malik for support of his study.en_US
dc.identifier.doi10.1016/j.jbiotec.2023.12.010
dc.identifier.endpage63en_US
dc.identifier.issn0168-1656
dc.identifier.issn1873-4863
dc.identifier.pmid38151110en_US
dc.identifier.scopus2-s2.0-85181806123en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage51en_US
dc.identifier.urihttps://doi.org/10.1016/j.jbiotec.2023.12.010
dc.identifier.urihttps://hdl.handle.net/20.500.12491/13252
dc.identifier.volume380en_US
dc.identifier.wosWOS:001153405200001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorSaba, Kiran
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofJournal of Biotechnologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmzYK_20240925en_US
dc.subjectAquacultureen_US
dc.subjectPlant-based vaccinesen_US
dc.subjectOmpKen_US
dc.subjectVibriosisen_US
dc.subjectSubunit vaccinesen_US
dc.subjectTobaccoen_US
dc.subjectTransformationen_US
dc.subjectComputational bioinformaticsen_US
dc.titleIn silico designing and characterization of outer membrane protein K (OmpK) from Vibrio anguillarum and its expression in Nicotiana tabacum for the development of a plant-based vaccine against fish vibriosisen_US
dc.typeArticleen_US

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