Genome-wide identification and expression analysis of DGK (Diacylglycerol Kinase) genes in common bean
Yükleniyor...
Dosyalar
Tarih
2023
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Springer
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Diacylglycerol kinases (DGKs) are a family of enzymes that play an important functional role in various stress responses in plants by converting diacylglycerol to phosphatidic acid. In this study, genome-wide identification of DGK genes, expression analysis under non-stress in various developmental stages and under salt stress in different tissues of common bean were performed for the first time. The 6 DGK gene family members (PvDGK1, 2, 3, 5a, 5b, and 6) having conserved catalytic domains were identified by an exploration of common bean genome via multiple online databases. The protein lengths and molecular weights of PvDGKs were found between 423 and 727 amino acids (aa) and ranging from 47.31 to 81.09 kDa, respectively. Phylogenetic analysis revealed that PvDGK genes grouped into three clusters as described for known plant DGK families; cluster I (PvDGK1, 2), cluster II (PvDGK3) and cluster III (PvDGK5a, 5b, 6) genes, respectively. Quantitative real-time PCR analysis revealed that all PvDGK genes indicated divergent expression patterns in all tissues at different developmental stages under non-stress condition. Furthermore, PvDGK genes were also significantly upregulated in response to salt stress in root and leaf tissues of common bean cultivars at different time points. These results indicated the possible roles of PvDGK genes in developmental stages and salt stress in common bean. The findings herein will guide further investigations on the comprehension and functional characterization of the PvDGKs and the breeding studies to be carried out related to stress in the common bean near future.
Açıklama
This research was funded by Bolu Abant Izzet Baysal University (Project ID: 2021.10.07.1518).
Anahtar Kelimeler
Abiotic Stress, Phosphatidic Acid, Gene Expression, Salt Stress, Phosphatidylinositol Monophosphate, Suspension-Culture
Kaynak
Journal of Plant Growth Regulation
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
42
Sayı
4
Künye
Yeken, M. Z., Özer, G., & Çiftçi, V. (2023). Genome-Wide Identification and Expression Analysis of DGK (Diacylglycerol Kinase) Genes in Common Bean. Journal of Plant Growth Regulation, 42(4), 2558-2569.
