Investigation of in vitro cytotoxicity and genotoxicity of wintergreen oil in rat primary neurons and N2a neuroblastoma cells

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dc.authorid0000-0003-3682-2068
dc.authorid0000-0002-7046-8990
dc.contributor.authorÇelik, Kübra
dc.contributor.authorTürkez, Hasan
dc.date.accessioned2021-06-23T19:44:06Z
dc.date.available2021-06-23T19:44:06Z
dc.date.issued2016
dc.departmentBAİBÜ, Tıp Fakültesi, Temel Tıp Bilimleri Bölümüen_US
dc.description.abstractWintergreen oil is obtained from the leaves of a Gaultheria procumbens and is commonly used as a folk remedy. On the other hand neuroblastoma is a childhood cancer of the sympathetic nervous system that originates from developing neural crest cells and is the second most common solid tumour. It was aimed to investigate the biological activity of Wintergreen oil on primary rat healthy neuron and N2a neuroblastoma cell in relation with concentration for the first time in the extent of this study. The effects of Wintergreen oil at various concentrations (0-400 mg/L) on cell proliferation were determined on both cell culture types by 3-(4,5 dimetylthiazol-2-yl)-2,5 diphenltetrazolium bromide assay. And total antioxidant capacity and total oxidative stress parameters were used for the assessments of in vitro oxidative effects. In addition, the genetic effect of Wintergreen oil on healthy and cancerous brain cells was assessed by single cell gel electrophoresis (or Comet assay). Our results clearly revealed that Wintergreen oil exhibited antioxidant activities at their low concentrations and cytotoxic at high concentrations. The mean values of the total scores of cells showing DNA damage (for Comet assay) was not found significantly different from the control values in both cell types. Again, Wintergreen oil showed weak anticarcinogenic activity against neuroblastoma cells. In conclusion, within the light of the findings of this in vitro study, it is possible to denote that Wintergreen oil has low potential in cancer prevention and therapy.en_US
dc.identifier.doi10.1080/0972060X.2014.983993
dc.identifier.endpage1350en_US
dc.identifier.issn0972-060X
dc.identifier.issn0976-5026
dc.identifier.issue6en_US
dc.identifier.scopus2-s2.0-85000578288en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1340en_US
dc.identifier.urihttps://doi.org/10.1080/0972060X.2014.983993
dc.identifier.urihttps://hdl.handle.net/20.500.12491/8917
dc.identifier.volume19en_US
dc.identifier.wosWOS:000392634900003en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorÇelik, Kübra
dc.language.isoenen_US
dc.publisherTaylor & Francis Ltden_US
dc.relation.ispartofJournal Of Essential Oil Bearing Plantsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectWintergreen Oilen_US
dc.subjectN2a Neuroblastomen_US
dc.subjectOxidative Statusen_US
dc.subjectCytotoxicityen_US
dc.subjectCell Cultureen_US
dc.subjectSingel Cell Gel Electrophoresisen_US
dc.titleInvestigation of in vitro cytotoxicity and genotoxicity of wintergreen oil in rat primary neurons and N2a neuroblastoma cellsen_US
dc.typeArticleen_US

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