Gynogenesis induction in sugar beet (beta vulgaris) improved by 6-benzylaminopurine (BAP) and synergized with cold pretreatment

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dc.authorid0000-0001-6262-2866en_US
dc.authorid0000-0003-2379-5363en_US
dc.authorid0000-0002-1205-268Xen_US
dc.authorid0000-0002-4005-2476en_US
dc.authorid0000-0001-5291-1824en_US
dc.contributor.authorPazuki, Arman
dc.contributor.authorAflaki, Fatemeh
dc.contributor.authorGürel, Ekrem
dc.contributor.authorErgül, Ali
dc.contributor.authorGürel, Songül
dc.date.accessioned2021-06-23T19:49:57Z
dc.date.available2021-06-23T19:49:57Z
dc.date.issued2018
dc.departmentBAİBÜ, Fen Edebiyat Fakültesi, Biyoloji Bölümüen_US
dc.description.abstractFor sugar beet (Beta vulgaris) breeding, producing homozygous lines through haploid and doubled haploid techniques are preferred over conventional and time-consuming methods. Doubled haploid sugar beet production necessitates inducing ovules to develop into haploid plants, referred to as gynogenesis. The protocol involves an interaction between cold pretreatment of six genotypes of sugar beet inflorescences at 4 degrees C for 1 week or more and 6-benzylaminopurine (BAP) concentrations (1 or 2 mg L-1) to increase the response rate of haploid embryo induction. Compared with freshly cultured ovules (6.49%), cold pretreatment for 1 week almost doubled the mean of haploid plantlet induction rate (11.3%), whereas pretreatment for more than 1 week was not as effective as the control. Addition of 2 mg L-1 BAP to the culture medium nearly doubled the induction rate of the cultured ovules (10.75%), followed by 1 mg L-1 BAP (7.78%) in comparison with hormone-free medium (5.69%). The highest gynogenesis rate (37.8%) was achieved when ovules were cultured on medium containing 2 mg L-1 BAP following 1-week cold pretreatment. This combination approximately tripled the mean total haploid embryo induction rate of all the genotypes to 16.3% in comparison with the control (5.74%). However, the addition of BAP resulted in vitrification proportionately. As a result, 2 mg L-1 BAP decreased the normal plantlet emergence (NPE) to one-third (7.59%) while 1 mg L-1 BAP had a moderate effect (NPE: 18.98%) in comparison with hormone-free treatment (NPE: 24.35%). The results indicate that the combination of cold pretreatment and BAP is very effective in inducing haploid plants from recalcitrant genotypes of sugar beet, but BAP can have both advantages and disadvantages.en_US
dc.identifier.doi10.1007/s12355-017-0522-x
dc.identifier.endpage77en_US
dc.identifier.issn0972-1525
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-85017468984en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage69en_US
dc.identifier.urihttps://doi.org/10.1007/s12355-017-0522-x
dc.identifier.urihttps://hdl.handle.net/20.500.12491/9662
dc.identifier.volume20en_US
dc.identifier.wosWOS:000419715300009en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorGürel, Ekrem
dc.language.isoenen_US
dc.publisherSpringer Indiaen_US
dc.relation.ispartofSugar Techen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectSugar Beeten_US
dc.subjectHaploiden_US
dc.subjectCold Pretreatmenten_US
dc.subjectBeta Vulgarisen_US
dc.subject6-benzylaminopurine (BAP)en_US
dc.titleGynogenesis induction in sugar beet (beta vulgaris) improved by 6-benzylaminopurine (BAP) and synergized with cold pretreatmenten_US
dc.typeArticleen_US

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