Revealing an Iranian isolate of tomato brown rugose fruit virus: Complete genome analysis and mechanical transmission

dc.authorid0000-0002-5836-8030en_US
dc.authorid0000-0002-5863-2591en_US
dc.contributor.authorEsmaeilzadeh, Fereshteh
dc.contributor.authorSantosa, Adyatma Irawan
dc.contributor.authorÇelik, Ali
dc.contributor.authorKoolivand, Davoud
dc.date.accessioned2024-07-31T06:16:53Z
dc.date.available2024-07-31T06:16:53Z
dc.date.issued2023en_US
dc.departmentBAİBÜ, Ziraat Fakültesi, Bitki Koruma Bölümüen_US
dc.descriptionWe would like to appreciate the University of Zanjan, Iran, for providing the equipment and laboratories necessary for this study.en_US
dc.description.abstractAn analysis of the complete genome sequence of a novel isolate of tomato brown rugose fruit virus (ToBRFV) obtained from tomatoes in Iran and named ToBRFV-Ir is presented in this study. Comprehensive phylogenetic analysis utilizing key viral proteins, including 126 KDa, 183 KDa, movement protein (MP), and coat protein (CP), as well as the complete genome sequence, classified ToBRFV-Ir and 65 isolates from GenBank into three distinct clades. Notably, genetic diversity assessment revealed relatively low variability among the isolates, irrespective of their geographical or clade affiliation. Natural selection analysis based on the complete genome sequence showed that dN/dS values were consistently <1, indicating the prevailing role of negative selection across all populations. Analyses using the Recombination Detection Program and SplitsTree found no evidence of recombination events or signals in the complete genome sequence of the tested isolates. Thus, these results suggest that the genetic composition of ToBRFV remains stable without significant genetic exchange or recombination events occurring. A simple arithmetic comparison of the patristic distances and dates suggested that the time to the most recent common ancestor (TMRCA) of the ToBRFV populations is approximately 0.8 up to 2.7 with the closest tobamoviruses. An evolutionary study of the tested isolates from various countries based on the complete genome suggests Peruvian ancestry. The ToBRF-Ir isolate was successfully transmitted through mechanical inoculations to Solanum lycopersicum and Nicotiana rustica. These findings shed light on the genetic dynamics and transmission mechanisms of ToBRFV, providing valuable insights into its molecular characteristics and potential spread among susceptible plant species.en_US
dc.description.sponsorshipWe would like to appreciate the University of Zanjan, Iran, for providing the equipment and laboratories necessary for this study.; University of Zanjan, Iranen_US
dc.identifier.citationEsmaeilzadeh, F., Santosa, A. I., Çelik, A., & Koolivand, D. (2023). Revealing an Iranian Isolate of Tomato Brown Rugose Fruit Virus: Complete Genome Analysis and Mechanical Transmission. Microorganisms, 11(10), 2434.en_US
dc.identifier.doi10.3390/microorganisms11102434
dc.identifier.endpage17en_US
dc.identifier.issn2076-2607
dc.identifier.issue10en_US
dc.identifier.pmid37894095en_US
dc.identifier.scopus2-s2.0-85175093240en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1en_US
dc.identifier.urihttp://dx.doi.org/10.3390/microorganisms11102434
dc.identifier.urihttps://hdl.handle.net/20.500.12491/12293
dc.identifier.volume11en_US
dc.identifier.wosWOS:001097932000001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorÇelik, Ali
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.relation.ispartofMicroorganismsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectExperimental Plant Hostsen_US
dc.subjectGenetic Ancestryen_US
dc.subjectMechanical Transmissionen_US
dc.subjectPhylogenyen_US
dc.subjectPopulation Geneticsen_US
dc.subjectSelection Constrainten_US
dc.titleRevealing an Iranian isolate of tomato brown rugose fruit virus: Complete genome analysis and mechanical transmissionen_US
dc.typeArticleen_US

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