Sensitive and selective detection of amitrole based on molecularly imprinted nanosensor
Yükleniyor...
Dosyalar
Tarih
2021
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wiley
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Herein, a surface plasmon resonance (SPR)-based nanosensor was fabricated usingmolecular imprinting technique for the selective and sensitive detection of amitrole, acommonly used highly toxic pesticide. For this purpose, polymeric nanofilms involvingN-methacryloyl-L-tryptophan methyl ester and ethylene glycol dimethacrylate whichare functional monomer and cross-linker, respectively, were prepared on the SPR chipssurface via the ultravoilet polymerization method. The nanofilms were characterized byseveral techniques such as ellipsometry, Fourier transform infrared-attenuated totalreflection, atomic force microscope and contact angle measurements. The kinetic ana-lyses for amitrole detection were examined through amitrole-imprinted and non-imprinted SPR sensor chips. The developed imprinted sensor showed high selectivity tothe amitrole molecule than similar molecules and a good linear relationship for the0.06-11.90 nM concentration range with a low limit of detection value of 0.037 nM.The high imprinting efficiency (I.F: 62.38) of the amitrole-imprinted SPR sensor wasdetermined by comparing it with the non-imprinted SPR sensor. The most suitablemodel of this sensor is the Langmuir adsorption model. To statistically assess the reus-ability of the sensor, intraday experiments were tested three times with five replicates.The relative standard deviation% value less than <1.5 indicates high reproducibility forboth sensor production and reproducibility of the method. The prepared sensor wasalso applied efficiently for the selective detection of the amitrole in spiked samples pre-pared from vegetables to evaluate the matrix effect. As a result, amitrole-imprinted SPRsensors have been estimated to be highly selective, fast responsive, easy to use, reus-able and sensitive in detecting amitrole in both natural source and aqueous solutions.Label-free amitrole detection was performed by an aminoacid-based SPR sensor fabri-cated without the need for complex coupling processes.
Açıklama
Scientific and Technological Research Council of Turkey(Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK))
Anahtar Kelimeler
Amitrole, Molecular Imprinting, Nanofilm, Plasmon Resonance Sensor, Spr Sensors, Biosensor
Kaynak
Journal of Molecular Recognition
WoS Q Değeri
Q3
Scopus Q Değeri
Q4
Cilt
34
Sayı
11
Künye
Çakır, O., Bakhshpour, M., Göktürk, I., Yılmaz, F., & Baysal, Z. (2021). Sensitive and selective detection of amitrole based on molecularly imprinted nanosensor. Journal of Molecular Recognition, 34(11), e2929.
