An efficient in vitro regeneration system for Bellis perennis L. and comparison of phenolic contents of field-grown and in vitro-grown leaves by LC-MS/MS

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dc.authorid0000-0001-5245-6294
dc.contributor.authorKarakaş, Fatma Pehlivan
dc.contributor.authorTürker, Arzu Uçar
dc.date.accessioned2021-06-23T19:34:28Z
dc.date.available2021-06-23T19:34:28Z
dc.date.issued2013
dc.departmentBAİBÜ, Fen Edebiyat Fakültesi, Biyoloji Bölümüen_US
dc.description.abstractBellis perennis L. (common daisy) is a medicinal plant in the family Asteraceae. It has been used in the treatment of common cold, wounds, rheumatism and inflammation in traditional medicine. A highly efficient and rapid regeneration system was developed for common daisy for the first time. Field-grown plants and in vitro-grown plantlets were compared in terms of their regeneration capacity. The field-grown plants were found to be much more responsive than the in vitro-grown plantlets when used as explant source. Leaf, pedicel and root explants obtained from field-grown plants, and leaf, petiole and root explants obtained from in vitro-grown plantlets were cultured on Murashige and Skoog's minimal organics medium (MSMO) with various plant growth regulator combinations. The best shoot proliferation was obtained with pedicel explants on media containing 0.5 mg l(-1) thidiazuron (TDZ) and 0.5 mg l(-1) indole-3-acetic acid (IAA). Plant regeneration was observed trough indirect organogenesis. Regenerated shoots were rooted on MSMO medium containing different concentrations of IAA, indole-3-butyric acid (IBA), naphthalene acetic acid (NAA), and 2,4-dichlorophenoxyacetic acid (2,4-D). IAA (1.0 mg l(-1)) was determined as the most effective auxin for rooting. Rooted explants were transferred to vermiculite in Magenta containers for acclimatization period. After 2 weeks, they were planted in to plastic pots containing potting soil and maintained in the plant growth room. Approximately 3 months after the transfer to room conditions, the flowering of the regenerated plants could be observed. Quantification of the chosen phenolics in dichloromethane (DCM) and methanol (MeOH) extracts of field-grown leaves and in vitro-grown leaves was performed using high-performance liquid chromatography coupled with tandem mass spectrometry (LC-ESI-MS/MS) method. (C) 2013 Elsevier B.V. All rights reserved.en_US
dc.identifier.doi10.1016/j.indcrop.2013.04.008
dc.identifier.endpage170en_US
dc.identifier.issn0926-6690
dc.identifier.issn1872-633X
dc.identifier.scopus2-s2.0-84877883640en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage162en_US
dc.identifier.urihttps://doi.org/10.1016/j.indcrop.2013.04.008
dc.identifier.urihttps://hdl.handle.net/20.500.12491/7523
dc.identifier.volume48en_US
dc.identifier.wosWOS:000320904300021en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorKarakaş, Fatma Pehlivan
dc.institutionauthorTürker, Arzu Uçar
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofIndustrial Crops And Productsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBellis Perennisen_US
dc.subjectCommon Daisyen_US
dc.subjectIn Vitro Regenerationen_US
dc.subjectLC-ESI-MS/MSen_US
dc.subjectPhenolicsen_US
dc.titleAn efficient in vitro regeneration system for Bellis perennis L. and comparison of phenolic contents of field-grown and in vitro-grown leaves by LC-MS/MSen_US
dc.typeArticleen_US

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