Reducing time in detection of Listeria monocytogenes from food by MALDI-TOF mass spectrometry

dc.authorid0000-0002-0565-1289en_US
dc.authorid0000-0003-0438-1130
dc.authorid0000-0003-3610-2706
dc.authorid0000-0001-5650-1320
dc.contributor.authorKarasu-Yalçın, Seda
dc.contributor.authorSöylemez-Milli, Nursel
dc.contributor.authorEren, Ömer
dc.contributor.authorEryaşar-Örer, Kübra
dc.date.accessioned2021-06-23T19:17:08Z
dc.date.available2021-06-23T19:17:08Z
dc.date.issued2020
dc.departmentBAİBÜ, Rektörlük, Bilimsel Endüstriyel ve Teknolojik Uygulama ve Araştırma Merkezien_US
dc.departmentBAİBÜ, Mühendislik Fakültesi, Gıda Mühendisliği Bölümü
dc.description.abstractIn this study, direct detection of L. monocytogenes from liquid culture and enrichment broths containing foods was investigated by using MALDI-TOF MS. For determining the sole effect of food constituents on detection and accuracy of identification in enrichment broths, sterile foods were used before the experiments with food. L. monocytogenes could be detected in BHI broth after 24 h of incubation. Detection period was determined as 18 h for 3 x 10(1) cfu/mL initial bacterial count in BHI broth containing sterile food. The period extended in ONE broth containing sterile garnish, which was 24 and 30 h for 3 x 10(1) and 1 cfu/mL inoculum, respectively. It was found that identification times in UHT milk were longer than that of canned garnish. In the experiments performed with foods having a specific microbiota; White cheese, iceberg lettuce, parsley and watermelon were used. Although no reliable identification was obtained by using White cheese, iceberg lettuce and parsley, L. monocytogenes could be detected in 24 h in the enrichment broth containing watermelon. Detection was achieved during a single step enrichment in a reduced time of 24 h for even 1 cfu/mL initial inoculum.en_US
dc.identifier.doi10.1007/s13197-020-04869-6
dc.identifier.endpage4109
dc.identifier.issn0022-1155
dc.identifier.issue11
dc.identifier.scopus2-s2.0-85093530892en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage4102
dc.identifier.urihttps://doi.org/10.1007/s13197-020-04869-6
dc.identifier.urihttps://hdl.handle.net/20.500.12491/5247
dc.identifier.volume58
dc.identifier.wosWOS:000582096600003en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorEryaşar-Örer, Kübra
dc.institutionauthorKarasu-Yalçın, Seda
dc.institutionauthorSöylemez-Milli, Nursel
dc.institutionauthorEren, Ömer
dc.language.isoenen_US
dc.publisherSpringer Indiaen_US
dc.relation.ispartofJournal Of Food Science And Technology-Mysoreen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectFoodborne Pathogenen_US
dc.subjectRapid Detectionen_US
dc.subjectMALDI-TOF MSen_US
dc.subjectListeria Monocytogenesen_US
dc.subjectEnrichmenten_US
dc.titleReducing time in detection of Listeria monocytogenes from food by MALDI-TOF mass spectrometryen_US
dc.typeArticleen_US

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