Bilimsel Endüstriyel ve Teknolojik Uygulama ve Araştırma Merkezi

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https://hdl.handle.net/20.500.12491/10831

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SOSA/SSN sensör ontoloji çerçevelerini kullanarak laboratuvar ortamlarında semantik tabanlı anomali tespiti
(Pamukkale University, 2023) Milli, Musa; Milli, Mehmet; Lakestani, Sanaz; Aktaş, Özlem
Günümüz modern dünyasında, laboratuvarlar okullarda, hastanelerde ve birçok kurumda, eğitim hayatının, iş hayatının ve gündelik yaşamın vazgeçilmez parçaları haline gelmiştir. Laboratuvarlar gerek eğitim alanında, gerek sağlık alanında veya gerekse endüstriyel alanda kullanılsın en temel prensip çalışanların ve çevrenin güvenliğinin sağlanması olmalıdır. Güvenlik önlemlerin ise en başında insan sağlığını doğrudan etkileyen ve laboratuvarların doğası gereği ortamda bulunmak zorunda olan fiziksel (sıcaklık, nem), kimyasal (gazlar), biyolojik (bakteriler, virüsler) ortam parametrelerinin sürekli izlenmesi, takibinin yapılması ve kontrol altında tutulması gelmektedir. Laboratuvar ortamlarında bu parametrelerin kontrol altında tutulması birçok yerde ya hiç yapılmamaktadır ya da hala klasik ve konvansiyonel yöntemler ile yapılmaktadır. Bu çalışmada laboratuvar ortam parametrelerinin devamlı izlenmesi amacı ile klasik yöntemlerin dezavantajlarını ortadan kaldırmak için sensör tabanlı bir sistem kurulmuştur. Önerilen sensör tabanlı sistem semantik web teknolojileri ile anlamsal olarak zenginleştirilmiştir. Böylelikle önerilen sistemin etkinliği ve sürdürülebilirliği de arttırılmıştır. Özellikle son yıllarda tüm dünyayı etkisi altına alan ve hava yolu ile bulaşan Covid-19 gibi hastalıkların yayılımın azaltmak için iç mekân ortamlarının hava kalitelerinin gözetimi ve iyileştirilmesi şarttır. Önerilen çalışmanın özellikle Covid-19 gibi salgın zamanlarında hastaneler, okullar, toplu taşıma araçları ve yoğun bakım üniteleri gibi kritik öneme sahip alanlarda kullanılma potansiyeli yüksektir. Sonraki çalışmalarda önerilen sisteme yapay zekâ yaklaşımları da eklenerek sisteme ileriye yönelik hava kalitesi tahmin kabiliyeti kazandırılacaktır. Geliştirilen sistem sayesinde kurumlar ve firmalar eylem planlarını daha erken devreye sokarak ortam şartlarının yönetilebilirliği noktasında avantaj sağlayacaklardır.
Equilibrium study of benzene, toluene, ethylbenzene, and xylene (BTEX) from gas streams by black pine cones-derived activated carbon
(Elsevier, 2023) Işınkaralar, Kaan; Türkyılmaz, Aydın; Lakestani, Sanaz
Considering environmental emissions, benzene, toluene, ethylbenzene, and xylene (BTEX) are widely used as raw materials in industrial processes. They also affect humans via inhalation, which must be reduced due to their toxicity before further operation. Notably, the leading technologies have tried to remove BTEX emissions with several methods. Continuous innovation of adsorbents is constantly developing in the adsorption mechanism, which has been developed based on waste biomass. Pinus nigra cones is a lignocellulosic raw material that is fast-growing on various soils and found abundant in nature as a precursor. It may be cheaply found available from some natural product vendors. It was used to prepare activated carbon by chemical activation with phosphoric acid (H3PO4), potassium hydroxide (KOH), sulfuric acid (H2SO4), lithium hydroxide (LiOH), zinc chloride (ZnCl2), sodium hydroxide (NaOH) as the activating agents between at 550-850 degrees C for 2 h. Fourier transforms infrared spectrometer (FT-IR), thermogravimetric analyzer (TGA), scanning electron microscopy (SEM), and N2 gas adsorption-desorption analyzer were used for KAS-ACs characterization. The high BTEX adsorption capacities by ZnCl2 activated carbons were slightly higher than (SBET: 1849 m2/g for KAS-AC91, and Vtotal: 0.44 cm3/g) others. ANOVA results show a high correlation for the KAS-ACs production with ZnCl2, and there was a statistically significant difference between the mean of Vmicro (cm3/g) with activation temperatures p-values<0.05. The removal capacities at 5 & mu;g/L have been done to evaluate using Tenax TA tubes were 92, 96, 88, and 94.08% for benzene, toluene, ethylbenzene, and xylene, respectively. The maximum adsorption capacity for benzene, toluene, ethylbenzene, and xylene onto the KAS-AC91 in the following order: Xylenes (181 & mu;g/g) > Toluene (206 & mu;g/g) >Benzene (171 & mu;g/g) >Ethylbenzene (201 & mu;g/g). This suggests that the KAS-AC91 is an efficient BTEX adsorbent and represents a promising attempt to enhance BTEX adsorption in indoor air quality. & COPY; 2023 The Author(s). Published by Elsevier B.V. This is an open access article under the CC
Comparison of the effects of surgical smoke on the air quality and on the physical symptoms of operating room staff
(SAGE Publications Inc, 2023) Soysal, Ganime Esra; İlçe, Arzu; Lakestani, Sanaz; Şit, Mustafa; Avcıoğlu, Fatma
Background Surgical smoke can be a hazard because e it contains toxic gases with carcinogenic effects that may threaten health. This study aims to determine the effect of surgical smoke containing toxic chemicals on indoor air quality and examine employees' physical symptoms in the operating room. Method The study was conducted in the operating room between June 2020 and July 2020. In the study, 45 air samples were taken before, during, and after surgery using the active sampling method. Nineteen employees working in the operating room were asked about their physical complaints and their throat cultures were taken before and after surgery. These results were compared with those of the employees working in internal units. Results The Total Volatile Organic Compounds value at the time of surgery was significantly higher (p <= 0.05). Benzene concentrations remained constantly high (p <= 0.05) throughout the surgery, exceeding the limit values. Other VOCs (Volatile Organic Compounds) were significantly higher during surgery and remained below the limit values (p <= 0.05). When compared in terms of open and laparoscopic surgery, no difference between VOC concentrations was observed (p <= 0.05). The physical symptoms of the surgical team increased during the operation, and they experienced more complaints of tearing, burning in the eyes, hair odor, nausea, and cough than those working in the internal units (e.g., internal medicine, dermatology; (p <= 0.05). Conclusions Surgical smoke was an important contaminant for indoor air quality in the operating room.
Monitoring, control and reduction of bioaerosols in the intensive care unit in Turkey
(Ice Publishing, 2022) Lakestani, Sanaz; Çakır, İbrahim; Gözdaş, Hasan Tahsin; Yıldız, İsa; Demirhan, Abdullah
Maintaining high indoor air quality in hospitals is important for protecting the health of both personnel and patients. In this study, the results of air quality monitoring carried out in the intensive care unit of a hospital in Bolu, Turkey, are discussed. Air disinfection devices were used to reduce the concentrations of bacteria. A 4-week sampling programme was implemented. In the first and the last weeks of the sampling, the disinfection devices were turned off, while in the second and third weeks, they were turned on. Bioaerosols were collected, and their indoor levels were determined by counting all plate colonies and bacterial types were identified by matrix-assisted laser desorption/ionisation-time of flight mass spectrometry. The highest levels of bacteria were measured in the mornings and before the air disinfection devices were turned on. Doctors did their rounds at approximately nine to ten o'clock. The dominant bacteria in the air samples examined were Staphylococcus hominis, Micrococcus luteus, Staphylococcus capitis, Staphylococcus epidermidis, Corynebacterium afermentans and Staphylococcus haemolyticus. The results showed that the air disinfection devices were effective in decreasing the concentration of microorganisms between 83.4 and 55% in the intensive care unit, which is very important for the healthcare staff and patients, particularly during pandemics.
Corrigendum to: The use of passive lichen biomonitoring in combination with positive matrix factor analysis and stable isotopic ratios to assess the metal pollution sources in throughfall deposition of Bolu plain, Turkey (vol 112, 106212, 2020)
(Elsevier, 2021) Dörter, Melike; Karadeniz, Hatice; Saklangıç, Uğur; Yenisoy, Serpil Karakaş
Düzeltme “The use of passive lichen biomonitoring in combination with positive matrix factor analysis and stable isotopic ratios to assess the metal pollution sources in throughfall deposition of Bolu plain, Turkey Orijinal makale:https://doi.org/10.1016/j.ecolind.2020.106212
Modelling sensor ontology with the SOSA/SSN frameworks: a case study for laboratory parameters
(Tubitak Scientific & Technical Research Council Turkey, 2020) Aktaş, Özlem; Milli, Mehmet; Lakestani, Sanaz; Milli, Musa
Recently, the use of sensor-based systems in many areas has led to an exponential increase in the raw sensor data. However, the lack of neither syntactic nor semantic integrity between these sensor data limited their sharing, reusability, and interpretation. These inabilities can cause some problems. For example, different wireless sensor networks may not work together due to the subtle variations in their sensing methods, operating systems, syntax, and data structure. In recent years, to cope with these inabilities, the semantic sensor web approach, which enables us to enrich the meaning of sensor data, has been seen as the critical technology in solving these problems by some researchers. The primary purpose of this study is to create a laboratory environment parameters sensor ontology (LEPSO) that provides a standard data model for heterogeneous sensor data from different platforms by expanding semantic sensor networks (SSN). A case study was conducted using the real-time data collected from Bolu Abant izzet Baysal University, Scientific Industrial Technological Application and Research Center in order to demonstrate that the proposed LEPSO can be used in similar sensor-based applications. A series of semantic queries have been performed on the collected sensor data to evaluate the proposed sensor ontology. The results showed that sensor data, which are heterogeneous by nature, provide benefit results in sensor-based monitoring systems when enriched with semantic web technologies and ontologies. Besides, this study proves that the proposed semantic sensor ontology, which used the semantic sensor network framework, has the capability to provide a common infrastructure for many sensor-based applications. The proposed ontology has the potential to become a more comprehensive ontology by adding different platforms, different sensors, different environments such as school, factory. In the next study, it is aimed to expand the scope of this semantic sensor network, which is formed by including this ontology in the intensive care unit of a hospital.
The use of passive lichen biomonitoring in combination with positive matrix factor analysis and stable isotopic ratios to assess the metal pollution sources in throughfall deposition of Bolu plain, Turkey
(Elsevier, 2020) Dörter, Melike; Karadeniz, Hatice; Saklangıç, Uğur; Yenisoy, Serpil Karakaş
The main objective of this study was to determine the elemental content of (51 elements) and stable isotope (delta C-13, delta N-15) content of lichen, Xanthoria parietina. The results were evaluated according to seasonal changes, pollution source, and altitudes. The data indicated that there is no significant impact of seasonal variation in concentrations of most of the elements and isotopes. The uptake efficiency of Xanthoria parietina and pine needle was compared and it was found out that significantly higher amounts of metals were accumulated by Xanthoria parietina than by pine needles. The lowest delta C-13 and the highest delta N-15 values were obtained in pine needle in both seasons. Both delta C-13 and delta N-15 showed increasing (for summer) and decreasing (for winter) trends with a rise in an altitude. Source apportionment of elements was performed by using positive matrix factorization (PMF) and supported by G-score maps. As a result, six sources were determined for the area namely urban environment, lichen nutrient elements, contaminated soil, traffic and mixed soil, lichen metabolism and coal combustion. The concentration pollution maps and G-score maps of Pb and Ba were similar and this was an indication of the vehicle emissions. The elements As, Bi and Tl signed the coal combustion.
Correction and source apportionment of vehicle emission factors obtained from Bolu Mountain Highway Tunnel, Turkey
(Turkish Natl Committee Air Pollution Res & Control-Tuncap, 2020) Karşı, Melike Büşra Bayramoğlu; Berberler, Ercan; Berberler, Tuğçe; Aslan, Özge; Karakaş, Serpil Yenisoy; Karakaş, Duran
Total suspended particulate (TSP) samples were collected in the atmosphere of Bolu Mountain Highway Tunnel located on the axis of Edirne-Istanbul-Ankara on the main artery of Turkey, by using PUF samplers between 24 and June 29, 2018. Thirty-five major and trace metals, 11 water-soluble anions and cations, and OC and EC concentrations were determined at the entrance and the exit of the tunnel. Emission factor calculations were carried out with and without correction of the data set against crustal contributions. The non-crustal net concentration data set was used as an input for the factor analysis and factor analysis-multiple linear regression. Factor analysis identified five sources, namely, (1) road dust re-suspension, (2) road cover material and tyre wear abrasion, (3) brake pad solid lubricants, (4) diesel combustion emission, and (5) gasolineLPG combustion emission. Contributions of identified sources on the TSP concentrations and emission factors were quantified by using the FA-MR technique and the results were discussed. Tin and Sb were identified and reported as the best marker for the brake-related non-exhaust emissions in the road tunnel. FA-MR results showed that 46, 60, 49, and 37% of Ba, Cu, Fe, and Mn, respectively were contributed by dust re-suspension. The main sources of EC (39%) and OC (49%) were observed to be gasoline-LPG emissions and 69% of sulfate and 62% of Pb in TSP fraction was contributed by diesel emission. Tin and Sb were mainly affected by the brake pad solid lubricant factor and 64% of Sn and 53% of Sb were contributed by this factor.
Evaluation of hourly-based precipitation chemistry in suburban site of Bolu
(Turkish Natl Committee Air Pollution Res & Control-Tuncap, 2020) Karadeniz, Hatice; Karakaş, Serpil Yenisoy
Precipitation chemistry is important for understanding atmospheric chemistry and transportation mechanism for a region. Therefore, 6-h rain samples were collected manually on the Black Sea Region of Turkey between March 2019 and May 2019. Totally, 21 wet deposition samples were collected in 12 rainy days. The collected rain samples were analyzed by ion chromatography for ions, including Cl-, NO2-, NO3-, SO42-, Na+, Ca2+, Mg2+, K+, NH4+. Volume weighted mean (VWM) concentrations of ions were calculated and were in the order of Na+ > Ca2+ > Cl- > SO42-> NO3- > Mg2+ > K+ > NH4+ > NO2-. The pH values of individual precipitation varied from 4.70 to 8.07. Anion to cation ratio was 0.74 +/- 0.34 thus it was an indication of anion deficiency. A strong correlation between NH4+ and SO42-, NH4+ and NO3-, Ca2+ and SO42-, Ca2+, and NO3- was found in the samples due to the neutralization process. The back trajectory model was used to find the long-range sources and the influence of air masses coming from Saharan, Europe, and the Black Sea. Calcium ion was found to be an effective ion in the neutralization processes by analysis of multiple linear regression and neutralization factor calculation. Approximately 79.5% of SO42- and 59.7% of NO3- were neutralized by Ca2+ and NH4+. The washout mechanism for Na+, Mg2+, Ca2+, and K+ was dominantly observed on the March 12, 2019. Rainout mechanism was seen on the April 13, 2019 rain event due to the long-range transportation from Saharan Dust.
Chitosan/poly(ethylene glycol)/hyaluronic acid biocompatible patches obtained by electrospraying
(Iop Publishing Ltd, 2018) Mutlu, Esra Cansever; Ficai, Anton; Ficai, Denisa; Yıldırım, Arzu Birinci; Yıldırım, Muhammet
Electrospray is a promising technique to scale-up production of microparticles and nanoparticles. In this study, electrospraying was used in order to produce candidate biopatches (CPH) by using chitosan, poly (ethylene glycol) (PEG) and hyaluronic acid (HA). Four different ratios of polymer blend compositions (CPH1, CPH2, CPH3 and CPH4) were tested by dissolving in 2% acetic acid solution (Ac.A.). The HA amount in each blend was kept the same to designate the optimum surface with different chitosan/PEG ratios for electrospray process. Fourier-transform infrared (FTIR) microscopy, scanning electron microscopy (SEM), and atomic force microscopy (AFM) studies showed that obtained patches had highly adhesive surfaces with the aid of heterogeneously distributed micro- and nano-particles. Additionally, video images of FTIR microscopy and AFM images proved that all surfaces have similar heterogeneity except CPH2. The most homogenous surface was obtained by CPH3. Patches were directly subjected to antibacterial tests against ten different types of gram positive and gram negative bacteria using disc diffusion assay (Kirby-Bauer method). Extraordinarily there was no antibacterial property of patches coated with microparticles. Finally, biocompatibility studies were performed by using mouse fibroblast L929 cell lines (ATTC number CCL-1) to test cell adhesion and proliferation properties of the patches. Results of 72 h viability tests proved the electrospray of ternary blends had displayed good biocompatibility; in particular, CPH3 had the highest cell viability.
Sugar based biopolymers in nanomedicine; new emerging era for cancer imaging and therapy
(Bentham Science Publ Ltd, 2017) Eroğlu, Mehmet Sayıp; Öner, Ebru Toksoy; Mutlu, Esra Cansever; Bostan, Müge Sennaroğlu
Since last decade, sugar based biopolymers are recognized in nanomedicine as promising materials for cancer imaging and therapy. Their durable, biocompatible and adhesive properties enable the fine tuning of their molecular weights (MW) and their miscellaneous nature makes the molecules acquire various conformations. These in turn provide effective endocytosis by cancer cell membranes that have already been programmed for internalization of different kinds of sugars. Therefore, biocompatible sugar based nanoparticles (SBNPs) are suitable for both cell-selective delivery of drugs and imaging through the human body. Recently, well known sugar-based markers have displayed superior performance to overcome tumor metastasis. Thereby, targeting strategies for cancer cells have been broadened to sugar-based markers as noticed in various clinic phases. In these studies, biopolymers such as chitosan, hyaluronic acid, mannan, dextran, levan, pectin, cyclodextrin, chondroitin sulphate, alginates, amylose and heparin are chemically functionalized and structurally designed as new biocompatible nanoparticles (NPs). The future cancer treatment strategies will mainly comprise of these multifunctional sugar based nanoparticles which combine the therapeutic agents with imaging technologies with the aim of rapid monitoring response to therapies. While each individual imaging and treatment step requires a long time period in effective treatment of diseases, these multifunctional sugar based nanoparticles will have the advantage of rapid detection, right drug efficiency evaluation and immediate interfere opportunity to some important diseases, especially rapidly progressing cancers. In this article, we evaluated synthesis, characterization and applications of main sugar based biopolymers and discussed their great promise in nano-formulations for cancer imaging and therapy. However much should be done and optimized prior to clinical applications of these nano-formulations for an efficient drug treatment without overall toxicity for getting most effective clinical results.
Statins and IL-1 beta, IL-10, and MPO levels in gingival crevicular fluid: preliminary results
(Springer/Plenum Publishers, 2016) Arı, Vuslat Çiçek; İlarslan, Yağmur Deniz; Erman, Baran; Sarkarati, Bahram; Tezcan, İlhan
Statins possess a wide variety of pleiotropic properties that are independent of their lipid-lowering abilities such as attenuating inflammation, oxidative stress, coagulation, platelet aggregation and stimulating bone formation. The aim of the study is to evaluate the effect of statins on clinical periodontal parameters and gingival crevicular fluid (GCF) levels of IL-1 beta, IL-10, and myeloperoxidase (MPO) in inflammatory periodontal diseases. Seventy-nine subjects with hyperlipidemia and 48 systemically healthy controls (C) were included. Hyperlipidemic patients were either given a diet (HD) or prescribed statin (HS). Patients were classified into three subgroups as those who were periodontally healthy (h), who had gingivitis (g), or who had chronic periodontitis (p). Blood samples were collected for the measurement of lipid profiles. Plaque index (PI), gingival index (GI), probing pocket depth (PD), clinical attachment level (CAL), and percentage of bleeding on probing (BOP) were recorded. Gingival crevicular fluid levels of IL-1 beta, IL-10, and MPO were measured in order to determine the anti-inflammatory and antioxidant effects of statins. Probing depth values of the HSp group were significantly lower than those of the Cp group. Percentage of BOP of the HSg group was significantly lower than those of the HDg and Cg groups. While the IL-1 beta level of the HSp group was significantly lower than that of the HDp group, IL-10 levels of the HSg group were significantly higher than those of the HDg group. MPO levels were significantly lower in the HSg group when compared to those in the HDg and Cg groups. Statin use decreased the IL-1 beta and MPO levels and enhanced IL-10 in GCF. It can be suggested that statins may attenuate periodontal inflammation and progression of periodontal inflammation.
Nitrite oxidation and tyrosine nitration by human myeloperoxidase
(Wiley-Blackwell, 2016) Sarkarati, Bahram; Kılınç, Kamer
Biomineralization is deposition of hydroxyapatite, the crystallized mineral form of calcium and phosphate, by cells to their extracellular matrix (ECM), and it is an essential mechanism of bone and teeth formation in humans. Biomineralization is especially important in adults for tissue regeneration in bone defects. ECM molecules regulate mineral formation and provide crystal growth and nucleation. One of the most important ECM molecules, Alkaline Phosphatase (ALP), is the key enzyme in biomineralization process by the activity of converting organophosphate into inorganic phosphate. Moreover, osteocalcin and osteopontin are small soluble noncollagenous proteins of ECM and they regulate biomineralization by binding to calcium atoms available at crystal surfaces due to their highly negative charged amino acid residues. In this study, ALP, osteocalcin and osteopontin are expressed in bacterial systems and purified to assess in vitro biomineralization. Optimization of in vitro biomineralization activities with osteocalcin and osteopontin proteins provided the understanding of the effect of protein concentrations in crystal structure of calcium crystals. Understanding of the effect of protein concentrations will provide control over biomineralization in different cell types by designing synthetic genetic circuits. Programming non-biomineral formation cells for biomineral formation will enable differentiation free bone mineral formation. Reprogramming of non-biomineral formation cells will help to treat bone defects and bone-impairing diseases, such as osteoporosis. Consequently, it is an outstanding approach to understand the activity of bone ECM proteins and construct synthetic genetic systems that can reprogram non-mineral formation cells for biomineralization within the scope of bone tissue engineering.
Lecithin-acrylamido-2-methylpropane sulfonate based crosslinked phospholipid nanoparticles as drug carrier
(Wiley, 2016) Mutlu, Esra Cansever; Bostan, Müge Sennaroğlu; Bahadori, Fatemeh; Koçyiğit, Abdurrahim; Öner, Ebru Toksoy
In this study, a novel paclitaxel (PTX) loaded and a crosslinked solid phospholipid nanoparticles (SLN-PTX) with negative surface charge was prepared by UV polymerization for drug delivery. Capping of positive charge of zwitterionic lecithin with negative charge of sodium 2-acrylamido-2-methyl-1-propanesulfonate (AMPS-Na) through cation exchange interaction produced a lecithin-AMPS (L-AMPS) complex. The amphiphilic and negative charged lipid complex was emulsified in the presence of emulsifier, paclitaxel, initiator, and methacrylated poly epsilon-caprolacton-diol (PCL-MAC) as a spacer. The colloidal system was subjected to UV-irradiation to obtain crosslinked nanoparticles. Completion of the UV-polymerization was monitored with differential scanning calorimetry (DSC), which indicated the disappearance of exothermic peaks of vinyl groups. The nanoparticle system, having an average size of 200 nm, exhibited high drug encapsulation (96%) with negatively charged surface (zeta potential had an average of -70 mV). PTX release profiles of the crosslinked and uncrosslinked SLN-PTXs were studied and their pharmacological properties were compared. The crosslinked nanoparticles exhibited more controlled release behavior with longer release time compared to the uncrosslinked ones. In vitro cytotoxicity test was conducted on MCF-7 human breast adenocarcinoma cell line, which indicated that the crosslinked SLN-PTXs have a potential therapeutic effect for breast cancer treatments. (c) 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016, 133, 44105.
An easy two-step purification method for human leucocyte myeloperoxidase
(Walter De Gruyter Gmbh, 2015) Sarkarati, Bahram; Akyol, Tülay Karaağaç; Kılınç, Kamer
Objective: The object of this study is to describe a simple, rapid and cost effective method for purification of human leucocyte myeloperoxidase from a single donor. Myelopeoxidase (MPO) was purified by a two step procedure consisting of concanavalin-A Sepharose 4B affinity chromatography followed by CM-Sephadex cation exchange chromatography. Methods: Leucocytes from a single donor collected by leucopheresis were used in purification studies. MPO was solubilized and extracted from leucocytes by homogenization in phosphate buffer containing 1% HETAB (hexadecyltrimethylammonium bromide). MPO containing soluble material was applied onto concanavalin-A Sepharose 4B affinity gel, and was eluted with methyl-a-D-manno-piranoside. Fractions with MPO activity were pooled, dialyzed and applied onto CM-sephadex cation exchange gel, and was eluted from the column at weak cationic pH with linear NaCl gradient. Results: By the use of two chromatographic procedures, MPO was purified from human leucocytes with 70% yield. Purity of MPO was checked by determining the Reinheit Zahl (RZ) value (A(430)/A(280)). The RZ value of 0.86 indicated that the purified enzyme was highly homogenous as compared to reported experimental values (ranging from 0.82 to 0.88) and pure commercial enzyme with the RZ value of 0.84. Conclusion: In comparison with earlier purification methods, the purification method reported here has higher recovery rate and high purity together. Use of leucocytes with leucopheresis origin help us to omit the leucocyte isolation step and omitting of ammonium sulphate precipitation steps also help us to reduce the cost and is shortened the time of purification.
Reducing time in detection of Listeria monocytogenes from food by MALDI-TOF mass spectrometry
(Springer India, 2020) Karasu-Yalçın, Seda; Söylemez-Milli, Nursel; Eren, Ömer; Eryaşar-Örer, Kübra
In this study, direct detection of L. monocytogenes from liquid culture and enrichment broths containing foods was investigated by using MALDI-TOF MS. For determining the sole effect of food constituents on detection and accuracy of identification in enrichment broths, sterile foods were used before the experiments with food. L. monocytogenes could be detected in BHI broth after 24 h of incubation. Detection period was determined as 18 h for 3 x 10(1) cfu/mL initial bacterial count in BHI broth containing sterile food. The period extended in ONE broth containing sterile garnish, which was 24 and 30 h for 3 x 10(1) and 1 cfu/mL inoculum, respectively. It was found that identification times in UHT milk were longer than that of canned garnish. In the experiments performed with foods having a specific microbiota; White cheese, iceberg lettuce, parsley and watermelon were used. Although no reliable identification was obtained by using White cheese, iceberg lettuce and parsley, L. monocytogenes could be detected in 24 h in the enrichment broth containing watermelon. Detection was achieved during a single step enrichment in a reduced time of 24 h for even 1 cfu/mL initial inoculum.
Anlamsal sensor verileri için bilgi yönetim istemi
(Institute of Electrical and Electronics Engineers, 2020) Aktaş, Özlem; Milli, Mehmet; Lakestani, Sanaz; Milli, Musa
The increasing number of wireless sensor networks, which are used extensively in industrial areas, has led to a tremendous increase in the data obtained from the sensors. This uncontrolled increase makes it difficult for managing and storing of data. Moreover, the lack of infrastructure to ensure the integrity of meaning between the data makes it impossible to share, reuse and interpret the data by machines. These inabilities can cause some problems like cannot managing together between separate wireless sensor networks due to the subtle variations in their sensing methods, operating systems, syntax, and data structure. To address all these problems, a semantic sensor network approach has been introduced which provides a common standard for sensor data and aims to enhance their meaning. In this study, semantic model with realworld usage from sensor networks measuring some laboratory parameters is proposed. In order to evaluate the proposed system, a series of semantic queries were prepared and applied to the obtained sensor data. The results show that sensor data can be managed on a common infrastructure using ontologies.
Yeniden birleştirilmiş proteinlerin proteaz kullanılmadan saflaştırılmasında intein aracılı ayırma süreçlerinin incelenmesi üzerine bir derleme
(2017) Ermurat, Yakup
Bu derleme çalışmasında, yeniden birleştirilmiş proteinlerin proteaz kullanılmadan saflaştırılması süreçlerinde yararlanılan inteinlerin ayrılma etkinliklerinin artırılması için inteinler ve hedef proteinler üzerinde yapılmış araştırmalar incelenmiştir. İntein kullanımı ile protein ayrışması üzerinde yapılan araştırmalar, intein ve protein saflaştırma süreçlerinin daha verimli hale getirilmesini amaçlamaktadır. In vivo ve in vitro olarak gerçekleşen süreçlerde, intein ve hedef protein içeren poliklonal Polimeraz Zincir Tepkimesi (PZT) ürününün çoğaltılması ve ekspres plazmidlerin kopyalanan suşlara taşınması işlemleri yapılmaktadır. İnteinin proteinden ayrılma etkinliği ve ayrılma oranı sabiti kinetikleri, değişik pH, sıcaklık ve süzme sürelerinde belirlenmekte ve hedef proteinlere ilişkili olarak karşılaştırılmaları yapılmaktadır. Araştırmalarda değişik doğal ve yapay inteinlerin protein ayrıştırma etkinlikleri incelenmiş ve yapay Nostoc punctiforme (Npu) DnaE C-inteinlerinin ayrışma etkinliklerinin en yüksek olduğu belirlenmiştir. Çalışmalar amino asit eklenmesine daha yatkın olması nedeniyle Cinteinler üzerinde yoğunlaşmaktadır.

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