Characterization of Dasypyrum villosum (L.) Candargy chromosomes by fluorescent in situ hybridization

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dc.authorid0000-0002-9131-4085
dc.contributor.authorUslu, Emel
dc.contributor.authorReader, Stephen M.
dc.contributor.authorMiller, Terry E.
dc.date.accessioned2021-06-23T18:54:57Z
dc.date.available2021-06-23T18:54:57Z
dc.date.issued1999
dc.departmentBAİBÜ, Fen Edebiyat Fakültesi, Biyoloji Bölümüen_US
dc.description.abstractFluorescent in situ hybridization (FISH) was utilized to study disomic wheat (Triticum aestivum)/Dasypyrum villosum chromosome addition lines to characterize the V genome chromosomes of D. villosum L. Candargy. The V genome specific DNA sequence, pHv62; the rye sequence, pSc119.2, the 18S-5.8S-26S rDNA genes, pTa71, and the 5S rDNA sequence, cP5S, were used as probes. Six of the seven added D. villosum chromosomes hybridized with pHv62. One of these, 3V, was shown to be a Robertsonian translocation with a wheat chromosome. The presence of the seventh addition line chromosome was verified by FISH using total genomic D. villosum DNA as probe. FISH with cP5S detected a site on 5V, whilst pTa71 revealed a single site on the short arm of 1V, but pSc119.2 detected sites on all the chromosomes of D. villosum. The pHv62 probe was also used in the application of FISH to a number of other species in the Triticeae, including five species of Aegilops, barley and the tetraploid Dasypyrum breviaristatum. Sites were detected only in Thinopyrum bessarabicum and Secale cereale, and this together with the above evidence for pTa71 and pSc119.2 suggests that D. villosum is phylogenetically closest to Th. bessarabicum and S. cereale, and also is not related to Dasypyrum breviaristatum.en_US
dc.identifier.doi10.1111/j.1601-5223.1999.00129.x
dc.identifier.endpage134en_US
dc.identifier.issn0018-0661
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-0033402851en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage129en_US
dc.identifier.urihttps://doi.org/10.1111/j.1601-5223.1999.00129.x
dc.identifier.urihttps://hdl.handle.net/20.500.12491/4631
dc.identifier.volume131en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorUslu, Emel
dc.language.isoenen_US
dc.publisherMendelian Society of Lunden_US
dc.relation.ispartofHereditasen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCharacterizationen_US
dc.subjectDasypyrum Villosum
dc.titleCharacterization of Dasypyrum villosum (L.) Candargy chromosomes by fluorescent in situ hybridizationen_US
dc.typeArticleen_US

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