Re-record and spore ultrastructure of nosema melasomae & Jodal 1986, a microsporidian pathogen of crysomela populi (coleoptera: chrysomelidae)
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Dosyalar
Tarih
2018
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Iranian Scientific Society Medical Entomology
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Background: Chrysomela (=Alelasoma) popu is one of the most serious pests on poplar plantations. In the present study, a microsporidian pathogen, Nosema melasomae infecting Crysomela poly is re-recorded from a new geographical locality and its spore ultrastructure is given for the first time. Methods: Larvae and adults of C. poly were dissected in Ringer's solution and prepared wet smears were examined under a microscope. Detected fresh and stained spores were measured and photographed using an Olympus BX51 microscope with a DP-25 digital camera and a DP2-BSW Soft Imaging System. The ultrastructure of the pathogen was studied with a Philips EM 208 transmission electron microscope using standard preparation techniques as previously described Results: Fresh spores of the microsporidian pathogen are elongate, 4.86 +/- .71 mu m in length and 1.64 +/- .19 mu min width. The spore wall is considerable thin, measured 60 to 100 nm and consists of a clear endospore (40 to 80 nm) and an electron-dense, uniform exospore (15 to 30 nm). The polar filament isisofilar and has only 6-8 coils. Nuclei in the cell are 400-560 nm in diameter. The polaroplast has a thin lamellated structure. Conclusion: The pathogen from C. poptili is N. melasomae Sidor & Jodal, 1986 and its systematic position given by Sidor and Jodal. The spore ultrastructure of N. melasomae differs from those of other microsporidia infecting chrysomelids.
Açıklama
Anahtar Kelimeler
Nosem a Melasomae, Sporeultrastructure, Microsporidium, Crysomela Populi, Chrysomelidae
Kaynak
Iranian Journal Of Parasitology
WoS Q Değeri
Q4
Scopus Q Değeri
Cilt
13
Sayı
2
