Protective effects of long-term probiotic mixture supplementation against pentylenetetrazole-induced seizures, inflammation and oxidative stress in rats

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dc.authoridKilinc, Erkan/0000-0001-9261-2634
dc.authoridAnkarali, Handan/0000-0002-3613-0523
dc.authoridankarali, seyit/0000-0003-3752-0846
dc.contributor.authorKilinc, Erkan
dc.contributor.authorAnkarali, Seyit
dc.contributor.authorAyhan, Didem
dc.contributor.authorAnkarali, Handan
dc.contributor.authorTorun, Ibrahim Ethem
dc.contributor.authorCetinkaya, Ayhan
dc.date.accessioned2024-09-25T19:57:48Z
dc.date.available2024-09-25T19:57:48Z
dc.date.issued2021
dc.departmentAbant İzzet Baysal Üniversitesien_US
dc.description.abstractEmerging evidence indicates that dysbiosis of gut microbiota plays an important role in epilepsy, although the underlying mechanisms remain unclear due to the complex nature of both microbial composition and pathophysiology of epilepsy. We investigated effects of long-term probiotics supplementation on epileptic seizures, and inflammatory and oxidant/antioxidant biomarkers in a pentylenetetrazole(PTZ)-induced seizure model in rats. Male Wistar weaner-rats were divided into four groups. The first two groups received 1 ml/day saline solution, while the other groups received 0.05 mg/1ml/day vehicle or 109cfu/1ml/day probiotic-mixture, respectively, for 60 days by gavage. Seizure was induced by a single convulsive dose of PTZ. Seizures were evaluated using Racine's scale. Concentrations of pro-inflammatory cytokines in plasma and brain tissue were determined using ELISA, while oxidant/antioxidant biomarkers were measured using an automated-colorimetric method. Probiotics supplementation exhibited anticonvulsant effects against PTZ-induced seizures by retarding onset-times of both myoclonic-jerk and generalized tonic-clonic seizure, and by shortening duration of generalized tonic-clonic seizure. Additionally, it alleviated PTZ-induced increases in levels of pro-inflammatory cytokines IL-1 beta, IL-6, and IL-17A, but not of IFN gamma, in plasma and brain tissue. Moreover, it restored PTZinduced fluctuations in levels of oxidants TOS and disulfide, and of antioxidants native thiol and total thiol. Our findings suggest that long-term probiotics supplementation exhibits protective effects against epileptic seizures, and alleviates (neuro)inflammation and oxidative stress related to pathophysiology of epilepsy. A probiotic-rich diet provided from childhood may provide prophylaxis against epileptic seizures, especially in susceptible individuals, as the neonate diet represents a fundamental extrinsic factor in establishing gut microbiota. (C) 2021 Elsevier Inc. All rights reserved.en_US
dc.description.sponsorshipBolu Abant Izzet Baysal Uni-versity Scientific Research Fund [2018.08.02.1370]en_US
dc.description.sponsorshipThis study was supported by the Bolu Abant Izzet Baysal Uni-versity Scientific Research Fund (Grant number 2018.08.02.1370) .en_US
dc.identifier.doi10.1016/j.jnutbio.2021.108830
dc.identifier.issn0955-2863
dc.identifier.issn1873-4847
dc.identifier.pmid34333116en_US
dc.identifier.scopus2-s2.0-85113143557en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1016/j.jnutbio.2021.108830
dc.identifier.urihttps://hdl.handle.net/20.500.12491/13590
dc.identifier.volume98en_US
dc.identifier.wosWOS:000703168900017en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Science Incen_US
dc.relation.ispartofJournal of Nutritional Biochemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmzYK_20240925en_US
dc.subjectEpilepsyen_US
dc.subjectMicrobiotaen_US
dc.subject(neuro)inflammationen_US
dc.subjectOxidative stressen_US
dc.subjectProbioticen_US
dc.titleProtective effects of long-term probiotic mixture supplementation against pentylenetetrazole-induced seizures, inflammation and oxidative stress in ratsen_US
dc.typeArticleen_US

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