Determination of yolk: White ratio of egg using SDS-PAGE

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Küçük Resim

Tarih

2020

Dergi Başlığı

Dergi ISSN

Cilt Başlığı

Yayıncı

Korean Society Food Science & Technology-Kosfost

Erişim Hakkı

info:eu-repo/semantics/openAccess

Özet

The aim of present study was to determine liquid egg adulteration based on yolk:white ratio of egg using sodium dodecyl sulphate-polyacrylamide gel electrophoresis and by measuring Brix. To construct a calibration model, liquid egg samples were prepared by mixing egg yolk and white (yolk:white) at different concentrations. A high coefficient of determination value (R-2 = 0.992) was obtained. Limit of detection and limit of quantification were estimated as 62 g/kg and 187 g/kg. The accuracy of the model was tested using eleven LWE samples, and the yolk ratios of 90.9% of these samples were predicted successfully. Liquid whole egg (LWE) containing additional egg white up to 30% was also predicted with a low relative error of less than 10%. Yolk:white ratio of LWE samples and authentication of the components of LWE (containing extra white or water) can be determined using proposed method.

Açıklama

Anahtar Kelimeler

Egg Adulteration, Authentication, Yolk, White Ratio, SDS-PAGE, Brix

Kaynak

Food Science And Biotechnology

WoS Q Değeri

Q3

Scopus Q Değeri

Q2

Cilt

29

Sayı

2

Künye