Enhancement of direct shoot regeneration and determination of bioactive secondary metabolites in leaves of Galega Officinalis L.

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dc.authorid0000-0001-5245-6294
dc.authorid0000-0003-0060-259X
dc.authorid0000-0001-9617-6673
dc.contributor.authorKarakaş, Fatma Pehlivan
dc.contributor.authorŞahin, Günce
dc.contributor.authorTürker, Arzu
dc.date.accessioned2021-06-23T18:40:10Z
dc.date.available2021-06-23T18:40:10Z
dc.date.issued2016
dc.departmentBAİBÜ, Ziraat Fakültesi, Tarla Bitkileri Bölümüen_US
dc.departmentBAİBÜ, Fen Edebiyat Fakültesi, Biyoloji Bölümü
dc.description.abstractGalega officinalis L. is a medicinal plant being used as a galactagogue and an antidiabetic in folk medicine. A new effective micropropagation protocol was established from leaf, stem, node, and root explants of G. officinalis for in vitro plant regeneration and callus induction systems. Murashige and Skoog (MS) medium supplemented with 55 various concentrations and 4 different combinations [benzyl adenine (BA)/naphthalene acetic acid (NAA), BA/indole-3-acetic acid (IAA), thidiazuron (TDZ)/indole-3-butyric acid (IBA), and TDZ/IAA] of plant growth regulators was used for the regeneration systems. The regenerated shoots were observed on only nodal explants through direct organogenesis at the 35th day. The highest shoot number (7.13 ± 1.12 shoots per explants) was obtained from nodal explants on MS medium supplemented with a 1.0/0.25 mg L-1 BA/NAA combination. The largest (2 cm in diameter) green and compact callus formations were induced using root explants on MS medium supplemented with 2.0/2.0 mg L-1 BA/NAA. A total of 16 and 11 phenolic compounds from wild-grown and in vitro-grown leaves was detected by using liquid chromatography-electrospray ionization-multistage/mass spectrometry (LC-ESI-MS/MS) analysis, respectively. Levels of apigenin, luteolin, and chlorogenic acid in in vitro-grown leaves were higher than those in wild-grown leaves. These findings showed that micropropagation of G. officinalis can be potentially used as a new protocol for the production of beneficial secondary metabolites in pharmaceutical and supplemental food industries.en_US
dc.identifier.endpage1319en_US
dc.identifier.issn1300-0152
dc.identifier.issn1303-6092
dc.identifier.issue6en_US
dc.identifier.scopus2-s2.0-85007324445en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1311en_US
dc.identifier.trdizinid214786en_US
dc.identifier.urihttps://app.trdizin.gov.tr/makale/TWpFME56ZzJOZz09
dc.identifier.urihttps://hdl.handle.net/20.500.12491/2997
dc.identifier.volume40en_US
dc.identifier.wosWOS:000391393100021en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.institutionauthorKarakaş, Fatma Pehlivan
dc.institutionauthorŞahin, Günce
dc.institutionauthorTürker, Arzu
dc.language.isoenen_US
dc.relation.ispartofTurkish Journal of Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectMicropropagationen_US
dc.subjectPlant Growth Regulators
dc.subjectCallus
dc.subjectPhenolic Compounds
dc.subjectLC-ESI-MS/MS
dc.titleEnhancement of direct shoot regeneration and determination of bioactive secondary metabolites in leaves of Galega Officinalis L.en_US
dc.typeArticleen_US

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