The association between sodium citrate cotransporter (NaDC-1) gene polymorphism and urinary citrate excretion in patients with calcium-containing kidney stones
Küçük Resim Yok
Tarih
2023
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Galenos Publ House
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Objective: To evaluate the relationship between sodium citrate cotransporter (NaDC-1) gene polymorphism and urinary citrate excretion in patients with kidney stones containing calcium.
Materials and Methods: Between June 2009 and August 2011, stone materials obtained from patients treated for nephrolithiasis at the Urology Clinic were examined using X-ray diffraction, and patients with calcium-containing stones (calcium oxalate and calcium phosphate) were identified. Patients were divided into two groups based on their 24-hour urine citrate levels: (1) those with normal urine citrate levels and (2) hypocitraturia. To analyze the rs11567842 mutation in the NaDC-1 gene, their blood was collected in a Na-EDTA hemogram tube and stored at -40 degrees C. The genotypes of the cases were determined by analyzing the obtained genomic DNAs in real-time polymerase chain reaction.
Results: Ninety-six patients with calcium-containing nephrolithiasis were eligible for this study, 40 with normal urine citrate levels and 56 with hypocitraturia. The mean 24-hour urine citrate levels in the normal- and hypo-citraturia groups were 773 mg/1.73 m(2)/24 hours and 152 mg/1.73 m(2)/24 hours, respectively. Citrate measurements revealed a statistically significant difference between the two groups (p<0.001). Twenty-fourhour urine oxalate, magnesium, calcium, and uric acid levels did not differ significantly between the groups (all p>0.05). NaDC-1 gene rs11567842 homozygous mutation (GG genotypes) was detected in 4 (10%) of normocitraturia and 4 (7%) of hypocitraturia. The normocitraturia group had a higher mutation rate than the hypocitraturia group, but this difference was insignificant (p=0.618).
Conclusion: This study suggests that the NaDC-1 gene polymorphism does not cause hypocitraturia in calcium-containing kidney stones. Larger studies are needed to understand genetic disorders' impact on low urinary citrate excretion, with patient groups and healthy controls, and a standard diet.
Açıklama
Anahtar Kelimeler
Citrate, Hypocitraturia, NaDC-1, Polymorphism, Kidney Stone, Urolithiasis
Kaynak
Journal of Urological Surgery
WoS Q Değeri
Q4
Scopus Q Değeri
Cilt
10
Sayı
4
Künye
Caliskan, A., Memik, O., Duzenli, S., & Tekin, A. (2023). The Association Between Sodium Citrate Cotransporter (NaDC-1) Gene Polymorphism and Urinary Citrate Excretion in Patients with Calcium-containing Kidney Stones. Journal of Urological Surgery, 10(4), 290-295.
