Selective recognition of nucleosides by boronate affinity organic-inorganic hybrid monolithic column

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dc.authorid0000-0003-3260-1639
dc.contributor.authorEktirici, Sisem
dc.contributor.authorGöktürk, Ilgım
dc.contributor.authorYılmaz, Fatma
dc.contributor.authorDenizli, Adil
dc.date.accessioned2021-06-23T19:55:08Z
dc.date.available2021-06-23T19:55:08Z
dc.date.issued2021
dc.departmentBAİBÜ, Gerede Meslek Yüksekokulu, Kimya Ve Kimyasal İşleme Teknolojileri Bölümüen_US
dc.description.abstractBoronic acids are important ligands used to selectively recognize and enrich compounds containing cis-diol groups such as nucleosides. In the present study, organic-inorganic hybrid [POSS-MAH-BPA] monolithic column was prepared for the first time in the literature as a new boronate affinity system for the recognition of nucleosides. The selectivity of the [POSS-MAH-PBA] boronate affinity monolithic column for the recognition of cis-diol containing adenosine nucleoside from its analogue molecule of deoxyadenosine triphosphate, dATP, noncis-diol containing compound was investigated both by UV and HPLC studies. When the relative selectivity coefficients are compared, the [POSS-MAH-PBA] boronate affinity monolithic column is 4.25 times more selective for adenosine than [POSS-MAH] monolithic column. Besides, to determine endogenous nucleosides in biological fluids, which may serve as non-invasive cancer biomarkers, nucleosides were spiked into the urine solutions and passed through the [POSS-MAH-PBA] boronate affinity monolithic column, and the nucleosides were confirmed by HPLC. The adenosine recognition capability of the [POSS-MAH-PBA] boronate affinity monolithic column with an average enrichment factor of 48.9-fold was apparently superior to that of the [POSSMAH] monolithic column. Methacryl Polyhedral Oligomeric Silsesquioxanes (POSS-MA) with nano-sized stable 3-dimensional architectures provided the advantage of being used as an adsorbent for the monolithic structure by providing high surface area, 507.60 m(2)/g, and enabling vinyl groups to function with amino acid-based MAH monomers capable of providing electrons to coordinate PBA. Recovery results of more than 90% for adenosine showed that the [POSS-MAH-PBA] boronate affinity monolithic column could be a promising adsorbent for selective adsorption of cis-diol containing compounds such as nucleosides.en_US
dc.identifier.doi10.1016/j.jchromb.2020.122477
dc.identifier.issn1570-0232
dc.identifier.issn1873-376X
dc.identifier.pmid33383498en_US
dc.identifier.scopus2-s2.0-85098180451en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1016/j.jchromb.2020.122477
dc.identifier.urihttps://hdl.handle.net/20.500.12491/10737
dc.identifier.volume1162en_US
dc.identifier.wosWOS:000612823000013en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorYılmaz, Fatma
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofJournal Of Chromatography B-Analytical Technologies In The Biomedical And Life Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBoronate Affinity Chromatographyen_US
dc.subjectMethacryloyl-L-histidineen_US
dc.subjectNucleosidesen_US
dc.subjectPolyhedral Oligomeric Silsesquioxaneen_US
dc.subjectHybrid Monolithic Columnen_US
dc.titleSelective recognition of nucleosides by boronate affinity organic-inorganic hybrid monolithic columnen_US
dc.typeArticleen_US

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