Assessment of genetic diversity in naturally growing 29 Trifolium L. taxa from Bolu Province using RAPD and SSR markers

Basit Öğe Kaydı
dc.authorid0000-0003-1774-9889
dc.contributor.authorUslu, Emel
dc.contributor.authorErtuğrul, Gözde Damla
dc.contributor.authorBabaç, Mehmet Tekin
dc.date.accessioned2021-06-23T19:35:02Z
dc.date.available2021-06-23T19:35:02Z
dc.date.issued2013
dc.departmentBAİBÜ, Fen Edebiyat Fakültesi, Biyoloji Bölümüen_US
dc.description.abstractTwo molecular marker technologies, RAPD and SSR, were used to determine the genetic diversity of 29 taxa of Trifolium L. collected from Bolu Province. The analysis was carried out using 11 RAPD and 5 SSR markers. A total of 446 and 331 fragments were produced by RAPD and SSR markers, respectively. The amplification products were 300-3500 bp with RAPD and 125-3000 bp with SSR markers. These bands were scored as absence and presence for all taxa. Data obtained were used to estimate genetic similarity using Jaccard's coefficient, and dendrograms were constructed by the UPGMA method using either RAPD and SSR markers individually or as combined sets of data. The highest genetic similarity values obtained were 0.33 between T hirtum All. and T constantinapolitanum Ser. for RAPD and 0.49 between T medium L. var. medium and var. eriocalycinum Hausskn. for SSR. The dendrograms produced from combined sets Of RAPD and SSR data revealed 2 main clusters. One contained members of the sections Chronosemium Ser., Lotoidea L., and Vesicaria Ser., and the other had only the section Trifolium. The results suggest that the use of 2 different molecular markers gives the most reliable genetic data for Trifolium taxa.en_US
dc.identifier.doi10.3906/biy-1212-27
dc.identifier.endpage490en_US
dc.identifier.issn1300-0152
dc.identifier.issn1303-6092
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-84880951374en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage479en_US
dc.identifier.trdizinid146932en_US
dc.identifier.urihttps://doi.org/10.3906/biy-1212-27
dc.identifier.urihttps://hdl.handle.net/20.500.12491/7691
dc.identifier.volume37en_US
dc.identifier.wosWOS:000324281500013en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.institutionauthorUslu, Emel
dc.institutionauthorErtuğrul, Gözde Damla
dc.institutionauthorBabaç, Mehmet Tekin
dc.language.isoenen_US
dc.publisherTubitak Scientific & Technical Research Council Turkeyen_US
dc.relation.ispartofTurkish Journal Of Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectTrifoliumen_US
dc.subjectFabaceaeen_US
dc.subjectBoluen_US
dc.subjectTurkeyen_US
dc.subjectRAPDen_US
dc.subjectSSRen_US
dc.subjectGenetic Relationshipen_US
dc.titleAssessment of genetic diversity in naturally growing 29 Trifolium L. taxa from Bolu Province using RAPD and SSR markersen_US
dc.typeArticleen_US

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