Effects of etanercept, a tumor necrosis factor receptor fusion protein, on primary cell cultures prepared from intact human intervertebral disc tissue

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dc.authorid0000-0001-7735-0584en_US
dc.authorid0000-0002-8084-7855en_US
dc.authorid0000-0002-1224-442X
dc.authorid0000-0001-5590-0637
dc.authorid0000-0002-5412-8355
dc.contributor.authorÇalışkan, Tezcan
dc.contributor.authorŞirin, Duygu Yaşar
dc.contributor.authorKaraarslan, Numan
dc.contributor.authorYılmaz, İbrahim
dc.contributor.authorÖzbek, Hanefi
dc.contributor.authorKaya, Yasin Emre
dc.date.accessioned2021-06-23T19:51:23Z
dc.date.available2021-06-23T19:51:23Z
dc.date.issued2019
dc.departmentBAİBÜ, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümüen_US
dc.description.abstractThe aim of the present study was to investigate the effects of etanercept (ETA), a tumor necrosis factor (TNF) inhibitor, on human cell cultures prepared from intact intervertebral disc tissue. ETA is used as a treatment for cases of rheumatoid arthritis, psoriatic arthritis, axial spondyloarthritis and ankylosing spondylitis accompanied by moderate or severe joint pain. ETA was applied to primary cell cultures [annulus fibrosus and nucleus pulposus (NP) from intact intervertebral disc tissue]. Cell cultures without ETA treatment served as the control group. Morphological and quantitative molecular analyses of the two groups were performed. The number of viable cells and cell proliferation decreased in the ETA-treated cultures as compared with those in the control group. Furthermore, in the treatment group, the chondroadherin gene, an NP-specific marker, was not expressed after 24 h. By contrast, the cartilage oligo matrix protein was expressed 24, 48 and 72 h post-ETA treatment, while its expression was significantly lower than that in the control group. In addition, the expression of interleukin-1 beta, as well as matrix metallopeptidase-7 and -19, was markedly decreased. Overall, the cell proliferation and gene expression in the ETA-treated cells were significantly different from those in the control group (P<0.05). These results suggest that the treatment duration and dosage of TNF inhibitors, which are used to suppress active inflammation, should be considered in the clinical setting. These biological agents may delay the healing of intervertebral disc tissue damage by slowing cell proliferation and altering gene expression via anabolic and catabolic pathways.en_US
dc.identifier.doi10.3892/etm.2019.7559
dc.identifier.endpage76en_US
dc.identifier.issn1792-0981
dc.identifier.issn1792-1015
dc.identifier.issue1en_US
dc.identifier.pmid31258639en_US
dc.identifier.startpage69en_US
dc.identifier.urihttps://doi.org/10.3892/etm.2019.7559
dc.identifier.urihttps://hdl.handle.net/20.500.12491/9977
dc.identifier.volume18en_US
dc.identifier.wosWOS:000476606000009en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorKaya, Yasin Emre
dc.language.isoenen_US
dc.publisherSpandidos Publ Ltden_US
dc.relation.ispartofExperimental And Therapeutic Medicineen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCartilage Oligomatrix Proteinen_US
dc.subjectChondroadherinen_US
dc.subjectEtanercepten_US
dc.subjectIntact Intervertebral Disc Tissueen_US
dc.subjectInterleukin-1 Betaen_US
dc.subjectMatrix Metalloproteinasesen_US
dc.subjectPrimary Cell Cultureen_US
dc.titleEffects of etanercept, a tumor necrosis factor receptor fusion protein, on primary cell cultures prepared from intact human intervertebral disc tissueen_US
dc.typeArticleen_US

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