High-performance liquid chromatographic determination of a saponin from verbascum thapsus L.
| dc.contributor.author | Uçar Türker, Arzu | |
| dc.contributor.author | Camper, N. Dwight | |
| dc.contributor.author | Gürel, Ekrem | |
| dc.date.accessioned | 2021-06-23T18:54:39Z | |
| dc.date.available | 2021-06-23T18:54:39Z | |
| dc.date.issued | 2004 | |
| dc.department | BAİBÜ, Fen Edebiyat Fakültesi, Biyoloji Bölümü | en_US |
| dc.description.abstract | An extraction and analytical protocol for saponins were established for Verbascum thapsus L., a medicinal plant. Four different kinds of plant sample were analyzed; field-grown, in vitro cultured, commercially obtained leaves and field-grown capsules. A cleanup procedure with Octadecyl (C18) Solid Phase Extraction Column was used prior to HPLC analysis. Ilwensisaponin A was used as an external standard and digitoxin as an internal standard. C18 reverse phase column and gradient elutions (Acetonitrile with 0.1 % Orthophosphoric acid and Water with 0.1 % Ortho- phosphoric acid) were used for HPLC analysis. Commercially obtained leaves had a higher concentration of saponin (0.215 mg/g tissue) than other leaves (0.081–0.198 mg/g tissue) and capsule sample (0.016 mg/g tissue). © 2004 Taylor and Francis Group, LLC. | en_US |
| dc.identifier.doi | 10.1080/13102818.2004.10819230 | |
| dc.identifier.endpage | 59 | en_US |
| dc.identifier.issn | 1310-2818 | |
| dc.identifier.issue | 1 | en_US |
| dc.identifier.scopus | 2-s2.0-1242351927 | en_US |
| dc.identifier.scopusquality | Q3 | en_US |
| dc.identifier.startpage | 54 | en_US |
| dc.identifier.uri | https://doi.org/10.1080/13102818.2004.10819230 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.12491/4523 | |
| dc.identifier.volume | 18 | en_US |
| dc.indekslendigikaynak | Scopus | en_US |
| dc.institutionauthor | Uçar Türker, Arzu | |
| dc.institutionauthor | Gürel, Ekrem | |
| dc.language.iso | en | en_US |
| dc.relation.ispartof | Biotechnology and Biotechnological Equipment | en_US |
| dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
| dc.rights | info:eu-repo/semantics/openAccess | en_US |
| dc.subject | Chromatographic | en_US |
| dc.subject | Liquid | |
| dc.title | High-performance liquid chromatographic determination of a saponin from verbascum thapsus L. | en_US |
| dc.type | Article | en_US |
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