High-performance liquid chromatographic determination of a saponin from verbascum thapsus L.
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Dosyalar
Tarih
2004
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
An extraction and analytical protocol for saponins were established for Verbascum thapsus L., a medicinal plant. Four different kinds of plant sample were analyzed; field-grown, in vitro cultured, commercially obtained leaves and field-grown capsules. A cleanup procedure with Octadecyl (C18) Solid Phase Extraction Column was used prior to HPLC analysis. Ilwensisaponin A was used as an external standard and digitoxin as an internal standard. C18 reverse phase column and gradient elutions (Acetonitrile with 0.1 % Orthophosphoric acid and Water with 0.1 % Ortho- phosphoric acid) were used for HPLC analysis. Commercially obtained leaves had a higher concentration of saponin (0.215 mg/g tissue) than other leaves (0.081–0.198 mg/g tissue) and capsule sample (0.016 mg/g tissue). © 2004 Taylor and Francis Group, LLC.
Açıklama
Anahtar Kelimeler
Chromatographic, Liquid
Kaynak
Biotechnology and Biotechnological Equipment
WoS Q Değeri
Scopus Q Değeri
Q3
Cilt
18
Sayı
1
