Cardenolide estimation in callus-mediated regenerants of Digitalis lamarckii Ivanina (dwarf foxglove)

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dc.authorid0000-0001-6262-2866en_US
dc.authorid0000-0002-6786-3973
dc.contributor.authorYücesan, Buhara
dc.contributor.authorMüller-Uri, Frieder
dc.contributor.authorKreis, Wolfgang
dc.contributor.authorGürel, Ekrem
dc.date.accessioned2021-06-23T19:36:17Z
dc.date.available2021-06-23T19:36:17Z
dc.date.issued2014
dc.departmentBAİBÜ, Fen Edebiyat Fakültesi, Biyoloji Bölümüen_US
dc.description.abstractDigitalis cardenolides can regulate heart rhythms and are effective agents in cancer chemotherapy, in particular, for treating prostate and breast cancer. In this study, an optimized and efficient plant tissue culture protocol was established using callus cultures of Digitalis lamarckii Ivanina, commonly known as dwarf foxglove. Lamina explants developed callus when cultured on Linsmaier and Skoog (LS) medium containing different concentrations of 6-benzyladenine (BA; 4.4, 13.3, or 22.2 mu M) and alpha-naphthalene acetic acid (NAA; 2.7, 5.4, or 10.8 mu M). The highest incidence of callus formation (100%) was achieved on LS medium containing 13.3 mu M BA and 10.8 mu M NAA. Indirect shoot regeneration was achieved when the callus explants were cultured on LS medium supplemented with varying concentrations of BA (0.4, 1.1, or 2.2 mu M) and/or gibberellic acid (0.7 or 1.4 mu M) for 8 wk. Following the rooting of shoots on LS medium supplemented with either indole-3-acetic acid (ranging from 1.4 to 5.7 mu M) or NAA (1.3 to 5.2 mu M), lamina and petiole tissues of the 4-mo-old regenerated plants were compared for their cardenolide contents. Lamina extracts showed nearly three times higher cardenolide accumulation than petiole extracts. Of the cardenolides analyzed by reverse-phase high-performance liquid chromatography, neo-odorobioside G and glucogitoroside were abundant in lamina extracts (170.3 and 143.9 mg/kg dry weight, respectively). The regeneration protocol described in this study can be used for the in vitro production of certain cardenolides from D. lamarckii.en_US
dc.identifier.doi10.1007/s11627-013-9549-1
dc.identifier.endpage142en_US
dc.identifier.issn1054-5476
dc.identifier.issn1475-2689
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-84895093904en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage137en_US
dc.identifier.urihttps://doi.org/10.1007/s11627-013-9549-1
dc.identifier.urihttps://hdl.handle.net/20.500.12491/7964
dc.identifier.volume50en_US
dc.identifier.wosWOS:000332456700016en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorYücesan, Buhara
dc.institutionauthorGürel, Ekrem
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofIn Vitro Cellular & Developmental Biology-Planten_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectDigitalis Lamarckiien_US
dc.subjectCardenolidesen_US
dc.subjectCallus Culturesen_US
dc.subjectIn Vitro Regenerationen_US
dc.titleCardenolide estimation in callus-mediated regenerants of Digitalis lamarckii Ivanina (dwarf foxglove)en_US
dc.typeArticleen_US

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