Genetic diversity and pathogenicity of Rhizoctonia spp. isolates associated with red cabbage in Samsun (Turkey)
Yükleniyor...
Dosyalar
Tarih
2021
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
MDPI
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Abstract: A total of 132 Rhizoctonia isolates were recovered from red cabbage plants with root rot and
wirestem symptoms in the province of Samsun (Turkey) between 2018 and 2019. Based on the se quence analysis of the internal transcribed spacer (ITS) region located between the 18S and 28S
ribosomal RNA genes and including nuclear staining, these 124 isolates were assigned to multinucle ate Rhizoctonia solani, and eight were binucleate Rhizoctonia. The most prevalent anastomosis group
(AG) was AG 4 (84%), which was subdivided into AG 4 HG-I (81%) and AG 4 HG-III (3%), followed
by AG 5 (10%) and AG-A (6%), respectively. The unweighted pair group method phylogenetic tree
resulting from the data of 68 isolates with the inter-PBS amplification DNA profiling method based on
interspersed retrotransposon element sequences confirmed the differentiation of AGs with a higher
resolution. In the greenhouse experiment with representative isolates (n = 24) from AGs on red cab bage (cv. Rondale), the disease severity index was between 3.33 and 4.0 for multinucleate AG isolates
and ranged from 2.5 to 3.17 for AG-A isolates. In the pathogenicity assay of six red cabbage cultivars,
one isolate for each AG was tested using a similar method, and all cultivars were susceptible to AG 4
HG-I and AG 4 HG-III isolates. Redriver and Remale were moderately susceptible, while Rescue,
Travero, Integro, and Rondale were susceptible to the AG 5 isolate. The results indicate that the most
prevalent and aggressive AGs of Rhizoctonia are devastating pathogens to red cabbage, which means
that rotation with nonhost-crops for these AGs may be the most effective control strategy. This is
the first comprehensive study of Rhizoctonia isolates in red cabbage using a molecular approach to
assess genetic diversity using iPBS-amplified DNA profiling.
Açıklama
This study was founded by the Research and Development Unit (BAP) of Ondokuz Mayis University with the project no PYO.ZRT.1904.18.015, and by the Science Committee of the Ministry of Education and Science of the Republic of Kazakhstan (grant no. AP08855353) for RK.
Anahtar Kelimeler
Red Cabbage, Rhizoctonia Spp, Anastomosis Groups, Ipbs, Pathogenicity
Kaynak
Journal of Fungi
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
7
Sayı
3
Künye
Erper, I., Ozer, G., Kalendar, R., Avci, S., Yildirim, E., Alkan, M., & Turkkan, M. (2021). Genetic diversity and pathogenicity of Rhizoctonia spp. isolates associated with red cabbage in Samsun (Turkey). Journal of fungi, 7(3), 234.
