Effect of mercury, cadmium, nickel, chromium and zinc on kinetic properties of NADPH-cytochrome P450 reductase purified from leaping mullet (Liza saliens)

dc.contributor.authorBozcaarmutlu, Azra
dc.contributor.authorArınç, Emel
dc.date.accessioned2021-06-23T19:20:20Z
dc.date.available2021-06-23T19:20:20Z
dc.date.issued2007
dc.departmentBAİBÜ, Fen Edebiyat Fakültesi, Kimya Bölümüen_US
dc.description31st Congress of the Federation-of-European-Biochemical-Societies (FEBS) -- JUN 24-29, 2006 -- Istanbul, TURKEYen_US
dc.description.abstractInformation on the mechanism of metal ion inhibition of NADPH-cytochrome P450 reductase is limited. The purpose of the present paper was to elucidate in vitro effect of Hg(+2), Cd(+2), Ni(+2), Cr(+3) and Zn(+2) ions on the purified mullet NADPH-cytochrome P450 reductase. NADPH-cytochrome P450 reductase was purified from detergent-solubilized liver microsomes from leaping mullet (Liza saliens). All of the metal ions caused inhibition of the enzyme activity except Zn(+2). At 50 mu M metal concentration, Hg(+2) inhibited the cytochrome P450 reductase activity completely (100%), while, at the same concentrations, Cd(+2), Cr(+3) and Ni(+2) caused 66%, 65% and 37% inhibition, respectively. At 50 mu M metal concentration, Zn(+2) had no apparent effect on cytochrome P450 reductase activity. The IC(50) values of HgCl(2), CrCl(3), CdCl(2) and NiCl(2) were estimated to be 0.07 mu M, 24 mu M, 33 mu M and 143 mu M, respectively. Of the metal ions tested, Hg(+2) exhibited much higher inhibitory effect at lower concentrations, so it was evidently a more potent inhibitor than the others. All four metal ions displayed noncompetitive type of inhibition mechanism for the purified reductase as analyzed by Dixon plot. K(i) values of Hg(+2), Cr(+3), Cd(+2) and Ni(+2) were calculated from Dixon plots as 0.048 mu M, 18 mu M, 73 mu M and 329 mu M, respectively. (c) 2006 Elsevier Ltd. All rights reserved.en_US
dc.description.sponsorshipFederat European Biochem Socen_US
dc.identifier.doi10.1016/j.tiv.2006.10.002
dc.identifier.endpage416en_US
dc.identifier.issn0887-2333
dc.identifier.issue3en_US
dc.identifier.pmid17113746en_US
dc.identifier.scopus2-s2.0-33947357707en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage408en_US
dc.identifier.urihttps://doi.org/10.1016/j.tiv.2006.10.002
dc.identifier.urihttps://hdl.handle.net/20.500.12491/6097
dc.identifier.volume21en_US
dc.identifier.wosWOS:000246548200011en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorBozcaarmutlu, Azra
dc.language.isoenen_US
dc.publisherPergamon-Elsevier Science Ltden_US
dc.relation.ispartofToxicology In Vitroen_US
dc.relation.publicationcategoryKonferans Öğesi - Uluslararası - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectNoncompetitive Inhibitionen_US
dc.subjectLeaping mullet (Liza saliens)en_US
dc.subjectNADPH-cytochrome p450 Reductaseen_US
dc.subjectMercuryen_US
dc.subjectCadmiumen_US
dc.subjectNickelen_US
dc.subjectChromiumen_US
dc.subjectZincen_US
dc.titleEffect of mercury, cadmium, nickel, chromium and zinc on kinetic properties of NADPH-cytochrome P450 reductase purified from leaping mullet (Liza saliens)en_US
dc.typeConference Objecten_US

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