Effect of mercury, cadmium, nickel, chromium and zinc on kinetic properties of NADPH-cytochrome P450 reductase purified from leaping mullet (Liza saliens)
dc.contributor.author | Bozcaarmutlu, Azra | |
dc.contributor.author | Arınç, Emel | |
dc.date.accessioned | 2021-06-23T19:20:20Z | |
dc.date.available | 2021-06-23T19:20:20Z | |
dc.date.issued | 2007 | |
dc.department | BAİBÜ, Fen Edebiyat Fakültesi, Kimya Bölümü | en_US |
dc.description | 31st Congress of the Federation-of-European-Biochemical-Societies (FEBS) -- JUN 24-29, 2006 -- Istanbul, TURKEY | en_US |
dc.description.abstract | Information on the mechanism of metal ion inhibition of NADPH-cytochrome P450 reductase is limited. The purpose of the present paper was to elucidate in vitro effect of Hg(+2), Cd(+2), Ni(+2), Cr(+3) and Zn(+2) ions on the purified mullet NADPH-cytochrome P450 reductase. NADPH-cytochrome P450 reductase was purified from detergent-solubilized liver microsomes from leaping mullet (Liza saliens). All of the metal ions caused inhibition of the enzyme activity except Zn(+2). At 50 mu M metal concentration, Hg(+2) inhibited the cytochrome P450 reductase activity completely (100%), while, at the same concentrations, Cd(+2), Cr(+3) and Ni(+2) caused 66%, 65% and 37% inhibition, respectively. At 50 mu M metal concentration, Zn(+2) had no apparent effect on cytochrome P450 reductase activity. The IC(50) values of HgCl(2), CrCl(3), CdCl(2) and NiCl(2) were estimated to be 0.07 mu M, 24 mu M, 33 mu M and 143 mu M, respectively. Of the metal ions tested, Hg(+2) exhibited much higher inhibitory effect at lower concentrations, so it was evidently a more potent inhibitor than the others. All four metal ions displayed noncompetitive type of inhibition mechanism for the purified reductase as analyzed by Dixon plot. K(i) values of Hg(+2), Cr(+3), Cd(+2) and Ni(+2) were calculated from Dixon plots as 0.048 mu M, 18 mu M, 73 mu M and 329 mu M, respectively. (c) 2006 Elsevier Ltd. All rights reserved. | en_US |
dc.description.sponsorship | Federat European Biochem Soc | en_US |
dc.identifier.doi | 10.1016/j.tiv.2006.10.002 | |
dc.identifier.endpage | 416 | en_US |
dc.identifier.issn | 0887-2333 | |
dc.identifier.issue | 3 | en_US |
dc.identifier.pmid | 17113746 | en_US |
dc.identifier.scopus | 2-s2.0-33947357707 | en_US |
dc.identifier.scopusquality | Q2 | en_US |
dc.identifier.startpage | 408 | en_US |
dc.identifier.uri | https://doi.org/10.1016/j.tiv.2006.10.002 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12491/6097 | |
dc.identifier.volume | 21 | en_US |
dc.identifier.wos | WOS:000246548200011 | en_US |
dc.identifier.wosquality | Q2 | en_US |
dc.indekslendigikaynak | Web of Science | en_US |
dc.indekslendigikaynak | Scopus | en_US |
dc.indekslendigikaynak | PubMed | en_US |
dc.institutionauthor | Bozcaarmutlu, Azra | |
dc.language.iso | en | en_US |
dc.publisher | Pergamon-Elsevier Science Ltd | en_US |
dc.relation.ispartof | Toxicology In Vitro | en_US |
dc.relation.publicationcategory | Konferans Öğesi - Uluslararası - Kurum Öğretim Elemanı | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.subject | Noncompetitive Inhibition | en_US |
dc.subject | Leaping mullet (Liza saliens) | en_US |
dc.subject | NADPH-cytochrome p450 Reductase | en_US |
dc.subject | Mercury | en_US |
dc.subject | Cadmium | en_US |
dc.subject | Nickel | en_US |
dc.subject | Chromium | en_US |
dc.subject | Zinc | en_US |
dc.title | Effect of mercury, cadmium, nickel, chromium and zinc on kinetic properties of NADPH-cytochrome P450 reductase purified from leaping mullet (Liza saliens) | en_US |
dc.type | Conference Object | en_US |
Dosyalar
Orijinal paket
1 - 1 / 1
Yükleniyor...
- İsim:
- azra-bozcaarmutlu.pdf
- Boyut:
- 251.85 KB
- Biçim:
- Adobe Portable Document Format
- Açıklama:
- Tam metin/Full Text