De novo assembly and annotation of microalga tetradesmus obliquus transcriptome

dc.authorid0000-0001-8711-6169en_US
dc.authorid0000-0003-0097-1125en_US
dc.contributor.authorÜnüvar, Ömer Can
dc.contributor.authorÜnlü, Ercan Selçuk
dc.date.accessioned2023-11-14T05:53:30Z
dc.date.available2023-11-14T05:53:30Z
dc.date.issued2022en_US
dc.departmentBAİBÜ, Lisansüstü Eğitim Enstitüsü, Fen Bilimleri, Kimya Ana Bilim Dalıen_US
dc.descriptionThis study was funded by Bolu Abant Izzet Baysal University (Grant number: 2015.03.03.819).en_US
dc.description.abstractTetradesmus obliquus is a unicellular green microalga and considered as a potential source for biotechnological production of pigments such as lutein. No genome-related data is available for T. obliquus that would increase the ability to develop new approaches in biotechnological applications. We present the first transcriptome data for T. obliquus. The aim of this study is to provide a comprehensive transcriptome annotation and identification of conserved genes involved in lutein pigment biosynthesis in Tetradesmus obliquus cells by analyzing pooled RNA-Seq data. Next-Generation Sequencing was applied for the pooled cDNAs library prepared by combining the cell cultures collected from samples exposed to dark and high light intensity conditions. Transcripts were assembled by the de novo assembly approach. Trinotate software was used for functional annotation of assembled transcripts. We also carried out BLAST analysis comparing the transcriptome data against known lutein biosynthesis genes. The 49.15% of the assembled sequences were functionally annotated, providing a total of 21490 unigenes. Our data also revealed the transcript sequences for ten conserved genes required for lutein biosynthesis. The data produced in this study can be used for molecular approaches in biotechnological applications related to T. obliquus, such as increasing the yield of pigment production.en_US
dc.description.sponsorshipBolu Abant Izzet Baysal University [2015.03.03.819]en_US
dc.identifier.citationÜnüvar, Ö. C., & Ünlü, E. S. (2022). De Novo Assembly and Annotation of Microalga Tetradesmus obliquus Transcriptome. Turkish Journal of Fisheries and Aquatic Sciences, 22(11).en_US
dc.identifier.doi10.4194/TRJFAS21341
dc.identifier.endpage8en_US
dc.identifier.issn1303-2712
dc.identifier.issn2149-181X
dc.identifier.issue11en_US
dc.identifier.scopus2-s2.0-85134418963en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage1en_US
dc.identifier.urihttp://dx.doi.org/10.4194/TRJFAS21341
dc.identifier.urihttps://hdl.handle.net/20.500.12491/11831
dc.identifier.volume22en_US
dc.identifier.wosWOS:000825320200001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorÜnüvar, Ömer Can
dc.institutionauthorÜnlü, Ercan Selçuk
dc.language.isoenen_US
dc.publisherCentral Fisheries Research Instituteen_US
dc.relation.ispartofTurkish Journal of Fisheries and Aquatic Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - İdari Personel ve Öğrencien_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectDenovoassemblyen_US
dc.subjectBioinformaticsen_US
dc.subjectMicroalgaeen_US
dc.subjectMicrobialbiotechnologyen_US
dc.subjectLuteinen_US
dc.subjectLutein Productivityen_US
dc.titleDe novo assembly and annotation of microalga tetradesmus obliquus transcriptomeen_US
dc.typeArticleen_US

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