In vitrotissue culture protocol of ancient einkorn (Triticum monococcumssp.monococum) wheat via indirect shoot regeneration

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dc.authorid0000-0001-6262-2866
dc.authorid0000-0002-5677-2347
dc.contributor.authorÖrgeç, Mehmet
dc.contributor.authorVerma, Sandeep Kumar
dc.contributor.authorŞahin, Günce
dc.contributor.authorZencirci, Nusret
dc.contributor.authorGürel, Ekrem
dc.date.accessioned2021-06-23T19:55:14Z
dc.date.available2021-06-23T19:55:14Z
dc.date.issued2021
dc.departmentBAİBÜ, Fen Edebiyat Fakültesi, Biyoloji Bölümüen_US
dc.description.abstractEinkorn indirect shoot regeneration was obtained from leaf, coleoptile, and root explants cultured on full, 1/2, and 1/4 strength Murashige and Skoog (MS) medium containing 0.5 to 10 mg L(-1)2,4-dichlorophenoxyacetic acid (2,4-D). Calluses derived from coleoptile and root explants were cultured on full-strength MS medium supplemented with 0.5 to 5 mg L(-1)thidiazuron (TDZ) for 6 wk to induce plant regeneration. Only callus cultures derived from coleoptile explants regenerated shoots. For root induction, 1.0 to 10 mg L(-1)indole-3-acetic acid (IAA) was used for 4 wk. The highest rate of callus formation (93%) was from root explants cultured on full-strength MS medium with 4 mg L(-1 )2,4-D. Coleoptile explants had the highest callus formation (100%) after culture on full-strength MS medium with 3 to 6 mg L(-1)of 2,4-D. The highest indirect shoot regeneration (7.0 +/- 1.1 shoots produced per callus with a 67% shoot formation frequency) was from calluses derived from coleoptile explant cultured on full MS medium supplemented with 5 mg L(-1)of TDZ. Of the different IAA concentrations investigated for rooting, the greatest number of roots per explant (7.7 +/- 0.088 roots produced per regenerated shoot with a 100% root formation frequency) was observed on MS medium supplemented with 6 mg L(-1)IAA. This indirect shoot regeneration protocol for coleoptile-derived einkorn wheat callus will be useful for future wheat genetic and improvement studies.en_US
dc.identifier.doi10.1007/s11627-020-10122-8
dc.identifier.endpage151en_US
dc.identifier.issn1054-5476
dc.identifier.issn1475-2689
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-85092911135en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage143en_US
dc.identifier.urihttps://doi.org/10.1007/s11627-020-10122-8
dc.identifier.urihttps://hdl.handle.net/20.500.12491/10758
dc.identifier.volume57en_US
dc.identifier.wosWOS:000580407700001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorŞahin, Günce
dc.institutionauthorGürel, Ekrem
dc.institutionauthorÖrgeç, Mehmet
dc.institutionauthorZencirci, Nusret
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofIn Vitro Cellular & Developmental Biology-Planten_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectEinkorn Wheaten_US
dc.subjectCallusen_US
dc.subjectRegenerationen_US
dc.subjectColeoptileen_US
dc.subjectExplanten_US
dc.titleIn vitrotissue culture protocol of ancient einkorn (Triticum monococcumssp.monococum) wheat via indirect shoot regenerationen_US
dc.typeArticleen_US

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