Inhibitory effect of organic acids on human neutrophil Myeloperoxidase's peroxidation, chlorination, and nitration activities

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Küçük Resim

Tarih

2023

Dergi Başlığı

Dergi ISSN

Cilt Başlığı

Yayıncı

Walter de Gruyter GmbH

Erişim Hakkı

info:eu-repo/semantics/openAccess

Özet

Objectives Myeloperoxidase from polymorphonuclear leukocytes is an important enzyme in oxidative metabolism and has a key role in tissue injuries in oxidative stress and inflammatory conditions. Therefore, its inhibitors have become the focus of studies on new drug development in recent years. The aim of the study was to determine the inhibitory effect of organic acids on the peroxidation, chlorination, and nitration activities of myeloperoxidase.Methods Seven organic acids naturally abundant in plants were tested. Different activities of myeloperoxidase were measured in the presence of various amounts of organic acids, and inhibition rates and kinetic parameters were determined for each organic acid separately.Results All the organic acids examined had inhibitory effects on the different activities of myeloperoxidase. Comparison of the IC50 values obtained for peroxidation, chlorination, and nitration activities showed that oxalic acid was the strongest inhibitor of myeloperoxidase activity, while citric acid and succinic acid were the weakest.Conclusions The results suggested that all the organic acids examined are inhibitors of myeloperoxidase. In particular, oxalic acid and fumaric acid are popular candidates for drug development research. More studies are needed to determine the in vivo effects of organic acids and their effects in the treatment of disease.

Açıklama

This work was supported by the Scientific Research Projects Department of Bolu Abant Izzet Baysal University (grant numbers 2019.31.01.1412). The funding organization played no role in the study design; in the collection, analysis, and interpretation of data; in writing of the report; or in the decision to submit report for publication.

Anahtar Kelimeler

Antioxidant, Enzyme Inhibition, Myeloperoxidase, Natural Products, Inflammation, Oxidants

Kaynak

Turkish Journal of Biochemistry - Türk Biyokimya Dergisi

WoS Q Değeri

Q4

Scopus Q Değeri

Q4

Cilt

48

Sayı

5

Künye

Sarkarati, B. (2023). Inhibitory effect of organic acids on human neutrophil myeloperoxidase’s peroxidation, chlorination, and nitration activities. Turkish Journal of Biochemistry, 48(5), 485-491.