Application of recent advances in hydrodynamic methods for characterising mucins in solution

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dc.authorid0000-0002-0335-2162en_US
dc.authorid0000-0003-1764-5748en_US
dc.authorid0000-0002-5681-0593en_US
dc.authorid0000-0001-6951-1114en_US
dc.authorid0000-0002-7050-4082
dc.contributor.authorAlmutairi, Fahad M.
dc.contributor.authorHernandez Cifre, Jose-Gines
dc.contributor.authorAdams, Gary G.
dc.contributor.authorKök, Mehmet Şamil
dc.contributor.authorMackie, Alan R.
dc.date.accessioned2021-06-23T19:44:47Z
dc.date.available2021-06-23T19:44:47Z
dc.date.issued2016
dc.departmentBAİBÜ, Mühendislik Fakültesi, Gıda Mühendisliği Bölümüen_US
dc.description.abstractMucins are the primary macromolecular component of mucus-nature's natural lubricant-although they are poorly characterised heterogeneous substances. Recent advances in hydrodynamic methodology now offer the opportunity for gaining a better understanding of their solution properties. In this study a combination of such methods was used to provide increased understanding of a preparation of porcine intestinal mucin (PIM), MUC2 mucin, in terms of both heterogeneity and quantification of conformational flexibility. The new sedimentation equilibrium algorithm SEDFIT-MSTAR is applied to yield a weight average (over the whole distribution) molar mass of 7.1 x 10(6) g mol(-1), in complete agreement with size exclusion chromatography coupled with multi-angle light scattering (SEC-MALS), which yielded a value of 7.2 x 10(6) g mol(-1). Sedimentation velocity profiles show mucin to be very polydisperse, with a broad molar mass distribution obtained using the Extended Fujita algorithm, consistent with the elution profiles from SEC-MALS. On-line differential pressure viscometry coupled to the SEC-MALS was used to obtain the intrinsic viscosity [eta] as a function of molar mass. These data combined with sedimentation coefficient data into the global conformation algorithm HYDFIT show that PIM has a flexible linear structure, with persistence length L (p) similar to 10 nm and mass per unit length, M (L) similar to 2380 g mol(-1) nm(-1), consistent with a Wales-van Holde ratio of similar to 1.2 obtained from the concentration dependence of the sedimentation coefficient.en_US
dc.identifier.doi10.1007/s00249-015-1075-0
dc.identifier.endpage54en_US
dc.identifier.issn0175-7571
dc.identifier.issn1432-1017
dc.identifier.issue1en_US
dc.identifier.pmid26596272en_US
dc.identifier.scopus2-s2.0-84952863600en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage45en_US
dc.identifier.urihttps://doi.org/10.1007/s00249-015-1075-0
dc.identifier.urihttps://hdl.handle.net/20.500.12491/9045
dc.identifier.volume45en_US
dc.identifier.wosWOS:000367609800005en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorKök, Mehmet Şamil
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofEuropean Biophysics Journal With Biophysics Lettersen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectMucinen_US
dc.subjectMolar Massen_US
dc.subjectConformationen_US
dc.subjectNovel Hydrodynamic Methodsen_US
dc.titleApplication of recent advances in hydrodynamic methods for characterising mucins in solutionen_US
dc.typeArticleen_US

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