Identification and biocontrol potential of entomopathogenic nematodes and their endosymbiotic bacteria in apple orchards against the codling moth, cydia pomonella (l.) (lepidoptera: tortricidae)
Yükleniyor...
Dosyalar
Tarih
2022
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
MDPI
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
The codling moth, Cydia pomonella (L.) (Lepidoptera: Tortricidae), is one of the major pests
in pome fruit production worldwide. Heavy treatment of the larvae of C. pomonella with insecticides
triggered the development of resistance to many groups of insecticides. In addition, the increasing
concern about the adverse effects of synthetic insecticides on human health and the environment
has led to the development of sustainable and eco-friendly control practices for C. pomonella. The
entomopathogenic nematodes (EPNs) (Steinernema and Heterorhabditis spp.) and their endosymbionts
(Xenorhabdus and Photorhabdus spp.) represent a newly emerging approach to controlling a wide
range of insect pests. In the present study, field surveys were conducted in apple orchards to isolate
and identify EPNs and their endosymbionts and evaluate their insecticidal efficacy on the larvae of
C. pomonella. EPNs were isolated from 12 of 100 soil samples (12%). Seven samples were identified as
Steinernema feltiae (Filipjev, 1934) (Rhabditida: Steinernematidae), whereas five samples were assigned
to Heterorhabditis bacteriophora (Poinar, 1976) (Rhabditida: Heterorhabditidae). The pathogenicity
of the EPN species/isolates was screened on the last instar larvae of G. mellonella. The two most
pathogenic isolates from each EPN species were tested against fifth instar larvae of C. pomonella under
controlled conditions. The maximum mortality (100%) was achieved by all EPN species/isolates at a
concentration of 100 IJs/larva 96 h after treatment. The endosymbionts of selected H. bacteriophora
and S. feltiae species were identified as Photorhabdus luminescens subsp. kayaii and Xenorhabdus bovienii,
respectively. The mortality rates ranged between 25 and 62% when the fifth larval instar larvae of
C. pomonella were exposed to the treatment of cell-free supernatants of symbiotic bacteria. In essence,
the present survey indicated that EPNs and their symbiotic bacteria have good potential for biological
control of C. pomonella
Açıklama
Anahtar Kelimeler
Sustainable Control, Biological Control, Apple, Beneficial Nematodes, Entomopathogenic Bacteria
Kaynak
Insects
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
13
Sayı
12
Künye
Gümüşsoy, A., Yüksel, E., Özer, G., İmren, M., Canhilal, R., Amer, M., & Dababat, A. A. (2022). Identification and biocontrol potential of entomopathogenic nematodes and their endosymbiotic bacteria in apple orchards against the codling moth, Cydia pomonella (L.)(Lepidoptera: Tortricidae). Insects, 13(12), 1085.
