Biofilm formation, icaABCD genes and agr genotyping of Staphylococcus aureus from fish and ground beef
Küçük Resim Yok
Tarih
2021
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Akademiai Kiado ZRT
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
A total of 46 Staphylococcus aureus isolates from fish and ground beef were tested for the agr types, icaABCD genes, and biofilm formation at 12, 25 and 37 8 degrees C by the microtiter plate and the MTT assays. All isolates were positive for the icaABD genes, while 97.8% were positive for the icaC. All isolates produced biofilms at 37 and 25 8 degrees C, but 93.5% of them were also biofilm producers at 12 8 degrees C. There was no significant difference in biofilm formation between 25 and 37 8C using the crystal violet assay (P > 0.05). However, statistically significant differences were detected between 12 and 25 8 degrees C as well as 12 and 37 8 degrees C (P < 0.05). All isolates were significantly different in biofilm production by the MTT assay at all tested temperatures. Furthermore, a relationship between the presence of the icaABCD genes and biofilm formation was observed. The agr type I was the most prevalent (54.4%) among the isolates, followed by agr type II (41.3%) and agr type III (9.6%). In this study, the S. aureus isolates exhibited biofilm formation ability responsible for persistence of bacteria in foods, which may lead to food spoilage and human health problems.
Açıklama
Anahtar Kelimeler
Agr Typing, Biofilm, icaABCD, Meat, Staphylococcus Aureus
Kaynak
Acta Alimentaria
WoS Q Değeri
Q4
Scopus Q Değeri
N/A
Cilt
50
Sayı
2
Künye
Özdemir, F., Arslan, S., & Eken, C. B. (2021). Biofilm formation, icaABCD genes and agr genotyping of Staphylococcus aureus from fish and ground beef. Acta Alimentaria, 50(2), 170-179.
