Spectrophotometric determination of various polyanions with polymeric film optodes using microtiter plate reader

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dc.authorid0000-0002-7627-349Xen_US
dc.authorid0000-0001-9481-1354
dc.contributor.authorDürüst, Nedime
dc.contributor.authorMeyerhoff, Mark E.
dc.contributor.authorÜnal, Nazangül
dc.contributor.authorNaç, Sibel
dc.date.accessioned2021-06-23T19:27:47Z
dc.date.available2021-06-23T19:27:47Z
dc.date.issued2011
dc.departmentBAİBÜ, Fen Edebiyat Fakültesi, Kimya Bölümüen_US
dc.description.abstractPolycation-sensitive membrane optodes based on the chromoionophore 2',7'-dichlorofluorescein octadecylester (DCFOE) have previously been developed and used for determination of heparin via a titri metric method. In this study, it is shown that some other important polyanions such as PPS (pentosan polysulfate), DNA, xanthan, Na-alginate, and carrageenan (food additive) can also be readily determined by using DCFOE-basecl microtiter plate-format optodes (MPOs) and polycationic titrants that bind these polyanionic species. The optical sensors are prepared with poly(vinyl chloride) (PVC), polyurethane (PU), bis(2-ethylhexyl)sebacate (DOS), and 2',7'-dichlorofluorescein octadecylester (DCFOE) and exhibit reproducible and sensitive absorbance changes in response to the varying polycationic titrant concentrations. Three different polycations; protamine, poly-L-lysine and poly-L-arginine, are employed as titrants. The method has a detection limit of 1 mu g mL(-1), and a dynamic range of 1-40 mu g mL(-1). After the quantitative determinations are successfully demonstrated in buffered solutions, similar titrations are also performed in real samples. The method is validated by recovery studies in these samples. The average polyanion recoveries were quantitative [99.7(+/- 1.3) % for pastry cream with vanillin (protamine titrant); 100.4 (+/- 3.3) % for pastry gel with strawberry(PLA titrant), and 102.9(+/- 2.0) % for pastry gel with strawberry (PLL titrant)]. (C) 2011 Elsevier B.V. All rights reserved.en_US
dc.identifier.doi10.1016/j.aca.2011.05.007
dc.identifier.endpage112en_US
dc.identifier.issn0003-2670
dc.identifier.issue1en_US
dc.identifier.pmid21704764en_US
dc.identifier.scopus2-s2.0-79959492255en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage107en_US
dc.identifier.urihttps://doi.org/10.1016/j.aca.2011.05.007
dc.identifier.urihttps://hdl.handle.net/20.500.12491/6893
dc.identifier.volume699en_US
dc.identifier.wosWOS:000293152100015en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorDürüst, Nedime
dc.institutionauthorÜnal, Nazangül
dc.institutionauthorNaç, Sibel
dc.language.isoenen_US
dc.publisherElsevier Science Bven_US
dc.relation.ispartofAnalytica Chimica Actaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectPolyanionen_US
dc.subjectThickening Agenten_US
dc.subjectPolymeric Film Sensoren_US
dc.subjectChromoionophoreen_US
dc.subjectMicrotiter Plate-format Optodeen_US
dc.titleSpectrophotometric determination of various polyanions with polymeric film optodes using microtiter plate readeren_US
dc.typeArticleen_US

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