A novel study on bean common mosaic virus accumulation shows disease resistance at the initial stage of infection in Phaseolus vulgaris

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dc.authorid0000-0002-5836-8030en_US
dc.authorid0000-0001-8021-2844en_US
dc.authorid0000-0003-0490-371Xen_US
dc.authorid0000-0003-0547-9527en_US
dc.authorid0000-0002-3385-2520en_US
dc.contributor.authorÇelik, Ali
dc.contributor.authorEmiralioğlu, Orkun
dc.contributor.authorYeken, Mehmet Zahit
dc.contributor.authorÇiftçi, Vahdettin
dc.contributor.authorÖzer, Göksel
dc.contributor.authorKim, Yoonha
dc.contributor.authorBaloch, Faheem Shehzad
dc.date.accessioned2023-08-16T10:39:16Z
dc.date.available2023-08-16T10:39:16Z
dc.date.issued2023en_US
dc.departmentBAİBÜ, Ziraat Fakültesi, Bitki Koruma Bölümüen_US
dc.descriptionThis research was funded by Bolu Abant Izzet Baysal University (Project ID: 2022.10.06.1550) and Basic Science Research Program supported this research through the National Research Foundation of Korea (NRF), funded by the Ministry of Education (2019R1A6A1A11052070).en_US
dc.description.abstractAccurate and early diagnosis of bean common mosaic virus (BCMV) in Phaseolus vulgaris tissues is critical since the pathogen can spread easily and have long-term detrimental effects on bean production. The use of resistant varieties is a key factor in the management activities of BCMV. The study reported here describes the development and application of a novel SYBR Green-based quantitative real-time PCR (qRT-PCR) assay targeting the coat protein gene to determine the host sensitivity to the specific NL-4 strain of BCMV. The technique showed high specificity, validated by melting curve analysis, without cross-reaction. Further, the symptoms development of twenty advanced common bean genotypes after mechanical BCMV-NL-4 infection was evaluated and compared. The results showed that common bean genotypes exhibit varying levels of host susceptibility to this BCMV strain. The YLV-14 and BRS-22 genotypes were determined as the most resistant and susceptible genotypes, respectively, in terms of aggressiveness of symptoms. The accumulation of BCMV was analyzed in the resistant and susceptible genotypes 3, 6, and 9 days following the inoculation by the newly developed qRT-PCR. The mean cycle threshold (Ct) values showed that the viral titer was significantly lower in YLV-14, which was evident in both root and leaf 3 days after the inoculation. The qRT-PCR thus facilitated an accurate, specific, and feasible assessment of BCMV accumulation in bean tissues even in low virus titers, allowing novel clues in selecting resistant genotypes in the early stages of infection, which is critical for disease management. To the best of our knowledge, this is the first study of a successfully performed qRT-PCR to estimate BCMV quantification.en_US
dc.description.sponsorshipNational Research Foundation of Korea (NRF) - Ministry of Education; Bolu Abant Izzet Baysal University [2022.10.06.1550]; [2019R1A6A1A11052070]en_US
dc.identifier.citationÇelik, A., Emiralioğlu, O., Yeken, M. Z., Çiftçi, V., Özer, G., Kim, Y., ... & Chung, Y. S. (2023). A novel study on bean common mosaic virus accumulation shows disease resistance at the initial stage of infection in Phaseolus vulgaris. Frontiers in Genetics, 14, 1136794.en_US
dc.identifier.doi10.3389/fgene.2023.1136794
dc.identifier.endpage11en_US
dc.identifier.issn1664-8021
dc.identifier.pmid37021006en_US
dc.identifier.scopus2-s2.0-85151513750en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1en_US
dc.identifier.urihttp://dx.doi.org/10.3389/fgene.2023.1136794
dc.identifier.urihttps://hdl.handle.net/20.500.12491/11547
dc.identifier.volume14en_US
dc.identifier.wosWOS:000962300900001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorÇelik, Ali
dc.institutionauthorEmiralioğlu, Orkun
dc.institutionauthorYeken, Mehmet Zahit
dc.institutionauthorÇiftçi, Vahdettin
dc.language.isoenen_US
dc.publisherFrontiers Media SAen_US
dc.relation.ispartofFrontiers in Geneticsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBCMVen_US
dc.subjectReal-Time PCRen_US
dc.subjectSYBR Greenen_US
dc.subjectVein-Yellowing-Virusen_US
dc.subjectRT-PCRen_US
dc.subjectQuantitative Detectionen_US
dc.titleA novel study on bean common mosaic virus accumulation shows disease resistance at the initial stage of infection in Phaseolus vulgarisen_US
dc.typeArticleen_US

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