Molecular cloning and characterization of bile salt hydrolase from lactobacillus gasseri atcc 33323 strain

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dc.authorid0000-0001-6906-5257en_US
dc.contributor.authorBa, Ndeye Mareme
dc.contributor.authorÖztürk, Mehmet
dc.date.accessioned2021-06-23T19:50:15Z
dc.date.available2021-06-23T19:50:15Z
dc.date.issued2018
dc.departmentBAİBÜ, Fen Edebiyat Fakültesi, Biyoloji Bölümüen_US
dc.description.abstractBile salt hydrolase (BSH) active probiotic strains are being used in the treatment of hypercholesterolemia related diseases. Understanding the mechanism of action of the BSH of probiotics is crucial. Even though Lactobacillus gasseri is probably one of the most beneficial probiotics on the market, there is no information on the catalytic activity and substrate preferences of its BSH. This study aims to detect the substrate specificity of the BSH of Lb. gasseri ATCC 33323 strain, to determine the optimum temperature and pH of the BSH's activity by ninhydrin assay. To do so, the BSH gene from Lb. gasseri ATCC 33323 was cloned into Escherichia coli XL1-Blue strain, expressed and characterized in E. coli BLR(DE3). Results indicated that even though the recombinant BSH (rBSH) was able to hydrolyze the six major human bile salts, there was an obvious preference for the glycine-conjugated bile salts and the maximum activities of the rBSH were recorded.en_US
dc.identifier.doi10.1080/08905436.2018.1443822
dc.identifier.endpage111en_US
dc.identifier.issn0890-5436
dc.identifier.issn1532-4249
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-85048940083en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage95en_US
dc.identifier.urihttps://doi.org/10.1080/08905436.2018.1443822
dc.identifier.urihttps://hdl.handle.net/20.500.12491/9737
dc.identifier.volume32en_US
dc.identifier.wosWOS:000436080100002en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorÖztürk, Mehmet
dc.language.isoenen_US
dc.publisherTaylor & Francis Incen_US
dc.relation.ispartofFood Biotechnologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBile Salt Hydrolaseen_US
dc.subjectLactobacillus Gasserien_US
dc.subjectProbioticsen_US
dc.subjectSubstrate Preferenceen_US
dc.titleMolecular cloning and characterization of bile salt hydrolase from lactobacillus gasseri atcc 33323 strainen_US
dc.typeArticleen_US

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