Cation exchange/hydrophobic interaction monolithic chromatography of small molecules and proteins by nano liquid chromatography

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dc.authorid0000-0002-7692-0423en_US
dc.authorid0000-0001-7548-5741
dc.authorid0000-0003-3260-1639
dc.contributor.authorAydoğan, Cemil
dc.contributor.authorYılmaz, Fatma
dc.contributor.authorDenizli, Adil
dc.date.accessioned2021-06-23T19:34:31Z
dc.date.available2021-06-23T19:34:31Z
dc.date.issued2013
dc.departmentBAİBÜ, Gerede Meslek Yüksekokulu, Kimya Ve Kimyasal İşleme Teknolojileri Bölümüen_US
dc.description.abstractIn this study, vinyl phenyl boronic acid modified lauryl methacrylate-based monolithic column was successfully prepared for cation exchange/hydrophobic interaction monolithic chromatography of small molecules and proteins in nano LC. The polymeric mixture consisted of lauryl methacrylate, vinyl phenyl boronic acid as cation exchanger, ethylene dimethacrylate as cross-linker, polyethylene glycol and methanol as binary porogenic solvent, and azobisisobutyronitrile as initiator. The resulting monolith showed good permeability and mechanical stability. Different ratios of monomer and porogens were used for optimizing the properties of the column. The monolithic column performance with respect to hydrophobic and cation exchange interactions was assessed by the separation a series of alkyl benzenes and anilines, respectively. cis-Diol-containing compounds such as phenols were also utilized to evaluate the retention behaviors of the vinyl phenyl boronic acid modified monolithic column. The monolithic column showed cation exchange interactions in the separation of aniline compounds. Theoretical plate number up to 52 000 plates/m was successfully achieved. The prepared monolith was further applied to the proteins with different acetonitrile content.en_US
dc.identifier.doi10.1002/jssc.201300089
dc.identifier.endpage1692en_US
dc.identifier.issn1615-9306
dc.identifier.issn1615-9314
dc.identifier.issue11en_US
dc.identifier.pmid23559572en_US
dc.identifier.scopus2-s2.0-84878750840en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1685en_US
dc.identifier.urihttps://doi.org/10.1002/jssc.201300089
dc.identifier.urihttps://hdl.handle.net/20.500.12491/7541
dc.identifier.volume36en_US
dc.identifier.wosWOS:000319919900001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorYılmaz, Fatma
dc.language.isoenen_US
dc.publisherWiley-V C H Verlag Gmbhen_US
dc.relation.ispartofJournal Of Separation Scienceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCapillary Columnen_US
dc.subjectCation Exchangeen_US
dc.subjectHydrophobic Monolithen_US
dc.subjectNano LCen_US
dc.subjectVinyl Phenyl Boronic Aciden_US
dc.titleCation exchange/hydrophobic interaction monolithic chromatography of small molecules and proteins by nano liquid chromatographyen_US
dc.typeArticleen_US

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