Differential expression of CD34, S100, and c-Kit in interstitial cells of cajal in infantile hypertrophic pyloric stenosis - immunochemical study
Yükleniyor...
Dosyalar
Tarih
2009
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wroclaw Medical Univ
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Background. The pathogenesis of infantile hypertrophic pyloric stenosis (IHPS) is poorly understood although many hypotheses have been proposed. Objectives. Assessment whether the differential expression of c-Kit, CD34, and S100 may be involved in the development of IHPS. Material and Methods. Specimens from 14 infants with IHPS and seven control subjects were immunohistochemically stained for c-Kit, CD34, and S100. The numbers of CD34(+), S100(+), and c-Kit(+) cells in five random fields per specimen were compared via light microscopy (x200). Results. In normal pyloric tissue, specific and intense c-Kit immunoreactivity was observed in the muscle layers and moderate staining was observed around the myenteric plexus. In IHPS patients, c-Kit+ cells were either absent or markedly reduced around the myenteric plexus. In control and IHPS patients, CD34(+) cells were not observed around the myenteric plexus. In the vascular endothelium, moderate CD34 staining was observed in specimens from control subjects, whereas intense staining was observed for IHPS patients. In normal pyloric tissue, moderate S100 immunoreactivity was observed in the muscle layers and intense staining was observed in the myenteric plexus. In IHPS patients, few S100(+) cells were observed in the pyloric muscle layers and S100 immunoreactivity decreased markedly around the myenteric plexus. Conclusions. These results suggest that the numbers of c-Kit(+) and S100(+) cells are markedly decreased in the pyloric muscle layers and around the myenteric plexus in IHPS patients. Thus a lack of c-Kit and S100, but not CD34, expression may be a critical factor in the pathogenesis of IHPS and may serve as a useful prognostic tool in the treatment of this disease (Adv Clin Exp Med 2009, 18, 1, 33-39).
Açıklama
Anahtar Kelimeler
Infantile Hypertrophic Pyloric Stenosis (IHPS), CD34, S100, C-Kit, Immunohistochemistry
Kaynak
Advances In Clinical And Experimental Medicine
WoS Q Değeri
Q4
Scopus Q Değeri
Q1
Cilt
18
Sayı
1
