Genetic bottlenecks in Turkish okra germplasm and utility of iPBS retrotransposon markers for genetic diversity assessment

dc.authorid0000-0002-7470-0080en_US
dc.authorid0000-0001-6534-5286
dc.authorid0000-0002-8109-5245
dc.contributor.authorYıldız, Mehtap
dc.contributor.authorKoçak, Metin
dc.contributor.authorBaloch, Faheem Shehazd
dc.date.accessioned2021-06-23T19:42:29Z
dc.date.available2021-06-23T19:42:29Z
dc.date.issued2015
dc.departmentBAİBÜ, Ziraat Fakültesi, Tarla Bitkileri Bölümüen_US
dc.description.abstractLack of requisite genetic variation in Turkish okra has necessitated the use of different types of markers for estimating the genetic diversity and identifying the source of variation. Transposable elements, present abundantly in plant genomes, generate genomic diversity through their replication and are thus an excellent source of molecular markers. We hypothesized that inter-primer binding site (iPBS)-retrotransposons could be the source of variation because of their genome plasticity nature. In the present study, genetic diversity of 66 okra landraces was analyzed using iPBS-retrotransposon markers. iPBS-retrotransposons detected 88 bands with 40.2% polymorphism and an average of 6.8 bands per primer. Gene diversity and Shannon's information index ranged from 0.01 to 0.13 and 0.02 to 0.21 for iPBS-retrotransposons and from 0.06 to 0.46 and 0.14 to 0.65 for simple sequence repeat (SSR) markers, respectively. Polymorphism information content value for retrotransposons varied between 0.12 and 0.99, while that for SSR was from 0.52 to 0.81. Neighbor joining analysis based on retrotransposons and SSRs divided all the accessions into four clusters; however, SSR markers were more efficient in clustering the landraces based on their origin. Using the STRUCTURE software for determining population structure, and two populations (at the number of hypothetical subpopulations, K = 2) were identified among the landraces. Low genetic diversity in Turkish okra highlights the need for the introduction of plants from countries with greater genetic diversity for these crops. This study also demonstrates the utility and role of iPBS-retrotransposons, a dominant and ubiquitous part of eukaryotic genomes, for diversity studies in okra.en_US
dc.identifier.doi10.4238/2015.September.8.20
dc.identifier.endpage10602en_US
dc.identifier.issn1676-5680
dc.identifier.issue3en_US
dc.identifier.pmid26400290en_US
dc.identifier.scopus2-s2.0-84941357768en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage10588en_US
dc.identifier.urihttps://doi.org/10.4238/2015.September.8.20
dc.identifier.urihttps://hdl.handle.net/20.500.12491/8505
dc.identifier.volume14en_US
dc.identifier.wosWOS:000362421700020en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorBaloch, Faheem Shehazd
dc.language.isoenen_US
dc.publisherFunpec-Editoraen_US
dc.relation.ispartofGenetics And Molecular Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectPopulation Structureen_US
dc.subjectAbelmoschus Esculentusen_US
dc.subjectGenetic Diversityen_US
dc.subjectRetrotransposonsen_US
dc.subjectTurkish Landracesen_US
dc.titleGenetic bottlenecks in Turkish okra germplasm and utility of iPBS retrotransposon markers for genetic diversity assessmenten_US
dc.typeArticleen_US

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