Protective effects of quercetin in combination with donepezil against H2O2-induced oxidative stress in glioblastoma cells

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dc.authorid0000-0001-7830-8214en_US
dc.authorid0000-0002-1586-6179en_US
dc.authorid0000-0003-2108-2458en_US
dc.authorid0000-0002-0270-9350en_US
dc.contributor.authorAlbayrak, Ahmet
dc.contributor.authorKurt, Akif Hakan
dc.contributor.authorKılıçaslan, Derya
dc.contributor.authorÇelik, Mustafa
dc.contributor.authorÖztabağ, Cansu Kara
dc.contributor.authorDoğaner, Adem
dc.date.accessioned2023-08-18T11:35:49Z
dc.date.available2023-08-18T11:35:49Z
dc.date.issued2023en_US
dc.departmentBAİBÜ, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümüen_US
dc.descriptionKahramanmaras Sutcu Imam University(Kahramanmaras Sutcu Imam University)en_US
dc.description.abstractPolyphenols are natural antioxidants found in the human diet, which are known to have protective effects against neurodegenerative diseases by scavenging free radicals. Recently, combination therapies including cholinesterase (ChE) inhibitors and neuroprotective agents have been shown to be more efficacious in the prevention and treatment of Alzheimer's disease. Therefore, the purpose of this work was to assess the neuroprotective impact of the combination of quercetin, a major polyphenolic compound, and donepezil, a cholinesterase inhibitor, against hydrogen peroxide-induced oxidative damage of glioblastoma (U-118 MG) cells. Following pretreatment with quercetin and donepezil, the glioblastoma cell line was subjected to H2O2-induced oxidative stress damage via application of 250 mu MH2O2 and incubation for a period of 24 h. In this study, four test groups were selected for the investigation of quercetin and donepezil as protective agents against H2O2 oxidative stress. First, quercetin and donepezil were examined in terms of their effects on glioblastoma cell viability. Next, pre- and post-application of quercetin and donepezil were used to determine their concentrations effective against the H2O2-induced oxidative damage in the U-118 MG cell line. Finally, the effect of the combined application of quercetin and donepezil on the cell viability was investigated. Viability testing of the cell lines was carried out via the advanced XTT assay using sulfonated tetrazolium. As a result of testing, no protective or therapeutic effects of donepezil alone were observed on the cell damage caused by oxidative stress. On the other hand, quercetin and combination applications exhibited protective or therapeutic effects on the cell damage. Thus, the neuroprotective effect of quercetin and its combination with donepezil provide a new approach to the search for drugs in the clinical treatment of neurodegenerative diseases.en_US
dc.description.sponsorshipKahramanmaras Sutcu Imam University [2019/4 - 22D]en_US
dc.identifier.citationAhmet, A., Hakan, K. A., Derya, K., Mustafa, C., Cansu, O. K., & Adem, D. (2023). Protective Effects of Quercetin in Combination with Donepezil against H2O2-Induced Oxidative Stress in Glioblastoma Cells. Pharmaceutical Chemistry Journal, 56(12), 1577-1586.en_US
dc.identifier.doi10.1007/s11094-023-02830-3
dc.identifier.endpage1586en_US
dc.identifier.issn0091-150X
dc.identifier.issn1573-9031
dc.identifier.issue12en_US
dc.identifier.scopus2-s2.0-85150278153en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage1577en_US
dc.identifier.urihttp://dx.doi.org/10.1007/s11094-023-02830-3
dc.identifier.urihttps://hdl.handle.net/20.500.12491/11579
dc.identifier.volume56en_US
dc.identifier.wosWOS:000952951500001en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorKurt, Akif Hakan
dc.institutionauthorid0000-0003-2940-3172
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofPharmaceutical Chemistry Journalen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectQuercetinen_US
dc.subjectDonepezilen_US
dc.subjectAlzheimer's Diseaseen_US
dc.subjectOxidative Stressen_US
dc.subjectU-118 MG Glioblastomaen_US
dc.subjectAcetylcholinesterase Inhibitorsen_US
dc.titleProtective effects of quercetin in combination with donepezil against H2O2-induced oxidative stress in glioblastoma cellsen_US
dc.typeArticleen_US

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