Embryogenesis, plant regeneration and cardiac glycoside determination in Digitalis ferruginea subsp ferruginea L

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dc.authorid0000-0001-6262-2866en_US
dc.authorid0000-0002-9324-747Xen_US
dc.authorid0000-0002-6786-3973
dc.contributor.authorVerma, Sandeep Kumar
dc.contributor.authorYücesan, Buhara
dc.contributor.authorŞahin, Günce
dc.contributor.authorGürel, Ekrem
dc.date.accessioned2021-06-23T19:35:15Z
dc.date.available2021-06-23T19:35:15Z
dc.date.issued2014
dc.departmentBAİBÜ, Fen Edebiyat Fakültesi, Biyoloji Bölümüen_US
dc.description.abstractThe present study reports, for the first time, an efficient in vitro plant regeneration protocol for Digitalis ferruginea subsp. ferruginea L. (rusty foxglove). We have used different concentrations of gibberellic acid (GA(3)) on Murashige and Skoog (MS) medium to assess the germination frequency of seeds. High frequency of germination was achieved on MS medium with 1.0 mg l(-1) GA(3). 6-Benzylaminopurine (BAP) combined with alpha-naphtaleneacetic acid (NAA) or 2, 4-dichlorophenoxy acetic acid (2, 4-D) in the induction MS medium induced both somatic embryogensis and shoot organogenesis. The highest percentage of callus growth (85 %) was obtained when hypocotyl explants were cultured on MS medium containing 0.5 mg l(-1) 2, 4-D plus 1.0 mg l(-1) BAP. The maximum mean number of somatic embryos (7.3 +/- A 1.3 embryos) or shoots (12.0 +/- A 1.1 shoots) per callus was obtained when medium contained 0.25 mg l(-1) NAA plus 1.0 mg l(-1) BAP or 0.5 mg l(-1) NAA plus 2.0 mg l(-1) BAP. The regenerated shoots easily rooted on MS medium. Higher amounts of lanatoside C [13.2 +/- A 0.5 mg 100 g(-1) dry weight (dw)] and digoxin (2.93 +/- A 0.31 mg 100 g(-1) dw) accumulation were obtained when shoots were obtained by indirect regeneration. We also investigated derivatives of cardenolides, i.e., digitoxigenin (730 +/- A 180 mg 100 g(-1) dw), gitoxigenin (50 +/- A 20 mg 100 g(-1) dw) and digoxigenin (490 +/- A 170 mg 100 g(-1) dw) from natural samples.en_US
dc.identifier.doi10.1007/s11240-014-0562-9
dc.identifier.endpage634en_US
dc.identifier.issn0167-6857
dc.identifier.issn1573-5044
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-84911805280en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage625en_US
dc.identifier.urihttps://doi.org/10.1007/s11240-014-0562-9
dc.identifier.urihttps://hdl.handle.net/20.500.12491/7748
dc.identifier.volume119en_US
dc.identifier.wosWOS:000345083800017en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorGürel, Ekrem
dc.institutionauthorVerma, Sandeep Kumar
dc.institutionauthorYücesan, Buhara
dc.institutionauthorŞahin, Günce
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofPlant Cell Tissue And Organ Cultureen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectDigitalis Ferrugineaen_US
dc.subjectRusty Foxgloveen_US
dc.subjectSomatic Embryogenesisen_US
dc.subjectPlant Regenerationen_US
dc.subjectCardiac Glycosidesen_US
dc.titleEmbryogenesis, plant regeneration and cardiac glycoside determination in Digitalis ferruginea subsp ferruginea Len_US
dc.typeArticleen_US

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