Isolation and biophysical characterisation of bioactive polysaccharides from cucurbita moschata (butternut squash)

Basit Öğe Kaydı
dc.authorid0000-0002-0335-2162en_US
dc.authorid0000-0002-4882-0329en_US
dc.authorid0000-0002-7798-9692en_US
dc.authorid0000-0002-7050-4082
dc.contributor.authorJiwani, Shahwar Imran
dc.contributor.authorGillis, Richard B.
dc.contributor.authorBesong, David
dc.contributor.authorAlmutairi, Fahad
dc.contributor.authorErten, Tayyibe
dc.contributor.authorKök, Mehmet Şamil
dc.contributor.authorHarding, Stephen E.
dc.date.accessioned2021-06-23T19:54:00Z
dc.date.available2021-06-23T19:54:00Z
dc.date.issued2020
dc.departmentBAİBÜ, Mühendislik Fakültesi, Gıda Mühendisliği Bölümüen_US
dc.description.abstractCucurbits are plants that have been used frequently as functional foods. This study includes the extraction, isolation, and characterisation of the mesocarp polysaccharide ofCucurbita moschata. The polysaccharide component was purified by gel filtration into three fractions (NJBTF1, NJBTF2, and NJBTF3) of different molecular weights. Characterisation includes the hydrodynamic properties, identification of monosaccharide composition, and bioactivity. Sedimentation velocity also indicated the presence of small amounts of additional discrete higher molecular weight components even after fractionation. Sedimentation equilibrium revealed respective weight average molecular weights of 90, 31, and 19 kDa, with the higher fractions (NJBTF1 and NJBTF2) indicating a tendency to self-associate. Based on the limited amount of data (combinations of 3 sets of viscosity and sedimentation data corresponding to the 3 fractions), HYDFIT indicates an extended, semi-flexible coil conformation. Of all the fractions obtained, NJBTF1 showed the highest bioactivity. All fractions contained galacturonic acid and variable amounts of neutral sugars. To probe further, the extent of glycosidic linkages in NJBTF1 was estimated using gas chromatography-mass spectrometry (GCMS), yielding a high galacturonic acid content (for pectin polysaccharide) and the presence of fructans-the first evidence of fructans (levan) in the mesocarp. Our understanding of the size and structural flexibility together with the high bioactivity suggests that the polysaccharide obtained fromC. moschatahas the potential to be developed into a therapeutic agent.en_US
dc.identifier.doi10.3390/polym12081650
dc.identifier.issn2073-4360
dc.identifier.issue8en_US
dc.identifier.pmid32722155en_US
dc.identifier.scopus2-s2.0-85089494621en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.3390/polym12081650
dc.identifier.urihttps://hdl.handle.net/20.500.12491/10378
dc.identifier.volume12en_US
dc.identifier.wosWOS:000564820200001en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorKök, Mehmet Şamil
dc.language.isoenen_US
dc.publisherMdpien_US
dc.relation.ispartofPolymersen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCucurbita Moschataen_US
dc.subjectGCMSen_US
dc.subjectLevanen_US
dc.subjectPectinen_US
dc.subjectBioactivityen_US
dc.subjectHydrodynamicsen_US
dc.titleIsolation and biophysical characterisation of bioactive polysaccharides from cucurbita moschata (butternut squash)en_US
dc.typeArticleen_US

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim
İsim:
shahwar-imran-jiwani.pdf
Boyut:
1.49 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin/Full Text
İndir

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Gıda Mühendisliği Bölümü Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu