Start Codon Targeted (SCoT) markers for the assessment of genetic diversity in yeast isolated from Turkish sourdough

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dc.authorid0000-0003-2924-5434en_US
dc.authorid0000-0002-3385-2520en_US
dc.authorid0000-0001-7775-1871en_US
dc.contributor.authorAydın, Furkan
dc.contributor.authorÖzer, Göksel
dc.contributor.authorAlkan, Mehtap
dc.contributor.authorÇakır, İbrahim
dc.date.accessioned2023-10-26T08:50:02Z
dc.date.available2023-10-26T08:50:02Z
dc.date.issued2022en_US
dc.departmentBAİBÜ, Ziraat Fakültesi, Bitki Koruma Bölümüen_US
dc.descriptionFunding The authors would like to thank the Scientific and Technological Research Council of Turkey (TUBITAK) for its financial support under project code 121O580.en_US
dc.description.abstractMolecular markers are valuable tools for assessing the genetic variation in yeast. Here, we investigated the utility of SCoT markers for the genetic characterization of yeast strains at inter and intraspecies levels. A total of 345 endogenous yeast strains were isolated from 65 Type I sourdough samples collected from six different regions of Turkey. The seven SCoT primers produced 221 bands, of which 95.47% were polymorphic. Each primer could successfully differentiate species, supported by PIC and RP values. The ITS sequencing of isolates selected from the UPGMA dendrogram revealed that Saccharomyces cerevisiae predominated the microflora, followed by Kazachstania servazzii, K. humilis, Wickerhamomyces anomalus, Torulaspora delbrueckii, and Pichia kudriavzevii, respectively. The AMOVA revealed a high genetic variation between (49%) and within populations (51%) for S. cerevisiae. The high gene flow observed among S. cerevisiae populations suggests that it may have contributed to the geographical evolution of S. cerevisiae via the transportation of the sourdough samples. The different geographical origins were most likely to group separately on the UPGMA and PCoA. Saccharomyces cerevisiae strains from more distant populations generally displayed more significant genetic variation. SCoT markers can successfully be used alone or with the other existing DNA markers for DNA fingerprinting and analyzing the genetic variation between and within species.en_US
dc.identifier.citationAydın, F., Özer, G., Alkan, M., & Çakır, İ. (2022). Start Codon Targeted (SCoT) markers for the assessment of genetic diversity in yeast isolated from Turkish sourdough. Food Microbiology, 107, 104081.en_US
dc.identifier.doi10.1016/j.fm.2022.104081
dc.identifier.issn0740-0020
dc.identifier.issn1095-9998
dc.identifier.pmid35953177en_US
dc.identifier.scopus2-s2.0-85132512575en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttp://dx.doi.org/10.1016/j.fm.2022.104081
dc.identifier.urihttps://hdl.handle.net/20.500.12491/11794
dc.identifier.volume107en_US
dc.identifier.wosWOS:000861899700013en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorÖzer, Göksel
dc.institutionauthorAlkan, Mehtap
dc.institutionauthorÇakır, İbrahim
dc.language.isoenen_US
dc.publisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTDen_US
dc.relation.ispartofFood Microbiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.relation.tubitak121O580
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectSCoT-PCRen_US
dc.subjectDNA Markeren_US
dc.subjectGenetic Diversityen_US
dc.subjectITS Sequencingen_US
dc.subjectYeasten_US
dc.subjectSourdoughen_US
dc.titleStart Codon Targeted (SCoT) markers for the assessment of genetic diversity in yeast isolated from Turkish sourdoughen_US
dc.typeArticleen_US

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