SERS-based rapid assay for sensitive detection of Group A Streptococcus by evaluation of the swab sampling technique

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dc.authorid0000-0001-9989-6123en_US
dc.authorid0000-0003-3034-7665en_US
dc.authorid0000-0003-1186-4229en_US
dc.authorid0000-0002-6639-4212
dc.authorid0000-0002-1207-5814
dc.authorid0000-0003-1333-060X
dc.contributor.authorEryılmaz, Merve
dc.contributor.authorSoykut, Esra Acar
dc.contributor.authorÇetin, Demet
dc.contributor.authorBoyacı, İsmail Hakkı
dc.contributor.authorSuludere, Zekiye
dc.contributor.authorTamer, Uğur
dc.date.accessioned2021-06-23T19:51:29Z
dc.date.available2021-06-23T19:51:29Z
dc.date.issued2019
dc.departmentBAİBÜ, Yeniçağa Yaşar Çelik Meslek Yüksekokulu, Gıda İşleme Bölümüen_US
dc.description.abstractBeta-hemolytic, Group A Streptococcus pyogenes (GAS) is a life-threating pathogen and the reason for prominent disease, pharyngitis. The conventional analysis of GAS, gold standard, takes 48 hours and the related rapid tests lack in accuracy and sensitivity. In this study, firstly, the efficiency of swab sampling, which is a must in the GAS detection, was discussed with the proposed surface-enhanced Raman spectroscopy (SERS)-based batch assay and each step was controlled by the plate-counting method. Secondly, SERS-based lateral flow immunoassay (LFIA) test strips were constructed and the variation in the SERS intensity of 5,5'-dithiobis (2-nitrobenzoic acid) (DTNB) was observed. Thus, a linear correlation was found with a R-2 value of 0.9926 and the LOD was calculated to be 0.2 CFU mL(-1) of GAS which could be counted as one cell. The combination of the gold standard with the LFIA-SERS technique enabled the fast and accurate pathogen detection. In addition, GAS was quantified with paper-based test strips up to 100 CFU ml(-1) level of bacteria for the first time without any interference. Besides, this study was featured with the discussion of the whole cell and pretreated cell detection of pathogens with LFIAs. Therefore, this work enlightens the points that have never been discussed on pathogen detection with paper-based platforms.en_US
dc.identifier.doi10.1039/c9an00173e
dc.identifier.endpage3580en_US
dc.identifier.issn0003-2654
dc.identifier.issn1364-5528
dc.identifier.issue11en_US
dc.identifier.pmid31062777en_US
dc.identifier.scopus2-s2.0-85066396881en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage3573en_US
dc.identifier.urihttps://doi.org/10.1039/c9an00173e
dc.identifier.urihttps://hdl.handle.net/20.500.12491/9995
dc.identifier.volume144en_US
dc.identifier.wosWOS:000474061200015en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorSoykut, Esra Acar
dc.language.isoenen_US
dc.publisherRoyal Soc Chemistryen_US
dc.relation.ispartofAnalysten_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectSERS-baseden_US
dc.titleSERS-based rapid assay for sensitive detection of Group A Streptococcus by evaluation of the swab sampling techniqueen_US
dc.typeArticleen_US

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