European and American grapevines were successfully recovered from GFkV, GLRaV1, GLRaV2, and GLRaV3 viruses by a modified thermotherapy and shoot tip culture

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dc.authorid0000-0002-7448-9502en_US
dc.authorid0000-0003-3327-1651en_US
dc.authorid0000-0001-9797-7536en_US
dc.contributor.authorSağlam, Hayri
dc.contributor.authorSağlam, Özlem Çalkan
dc.contributor.authorGüler, Emrah
dc.contributor.authorAkbaş, Birol
dc.contributor.authorGürner, Üftade
dc.date.accessioned2024-06-07T11:30:03Z
dc.date.available2024-06-07T11:30:03Z
dc.date.issued2023en_US
dc.departmentBAİBÜ, Ziraat Fakültesi, Bahçe Bitkileri Bölümüen_US
dc.description.abstractThis study was carried out to determine the effectiveness of modified thermotherapy and shoot tip culture in eliminating grapevines from grapevine fleck virus (GFkV), and grapevine leafroll closterovirus (GLRaV1, GLRaV2, and GLRaV3) all of which are required for certified sapling production. The infected grapevine scions were transferred into 10-liter plastic containers filled with a 1:1 mixture of perlite and peat (v/v), rooted, and saplings were grown. For 40 days, the grapevines were subjected to a modified thermotherapy treatment that included 8 h of darkness at 60-70% relative humidity and 32 & DEG;C temperature and 16 h of light (4000-5000 lx) at 60-70% relative humidity and 40 & DEG;C temperature. The materials were subjected to shoot tip culture procedures after thermotherapy. All materials were tested again at the end of the acclimatization period. The entire grapevines were virus-free at the end of the process. The survival rates of the grapevines varied. The 41B rootstock had a survival rate of 100%, and the rest had at least one surviving plant out of six. The proposed thermotherapy approach in this study has a high potential for sanitary grapevine sapling propagation when combined with rapid multiplication via in-vitro.en_US
dc.identifier.citationSağlam, H., Çalkan Sağlam, Ö., Güler, E., Akbaş, B., & Güner, Ü. (2023). European and American grapevines were successfully recovered from GFkV, GLRaV1, GLRaV2, and GLRaV3 viruses by a modified thermotherapy and shoot tip culture. Phytoparasitica, 51(4), 855-864.en_US
dc.identifier.doi10.1007/s12600-023-01101-x
dc.identifier.endpage864en_US
dc.identifier.issn0334-2123
dc.identifier.issn1876-7184
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-85168126270en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage855en_US
dc.identifier.urihttp://dx.doi.org/10.1007/s12600-023-01101-x
dc.identifier.urihttps://hdl.handle.net/20.500.12491/12210
dc.identifier.volume51en_US
dc.identifier.wosWOS:001049148600001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorGüler, Emrah
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofPhytoparasiticaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectGrapevineen_US
dc.subjectVirusen_US
dc.subjectThermotherapyen_US
dc.subjectShoot Tip Cultureen_US
dc.subjectIn-Vitroen_US
dc.subjectAssayen_US
dc.titleEuropean and American grapevines were successfully recovered from GFkV, GLRaV1, GLRaV2, and GLRaV3 viruses by a modified thermotherapy and shoot tip cultureen_US
dc.typeArticleen_US

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