Inactivation of Penicillum expansum in sour cherry juice, peach and apricot nectars by pulsed electric fields
Yükleniyor...
Tarih
2008
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Academic Press Ltd Elsevier Science Ltd
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Inhibitory effects of pulsed electric fields (PEF) on Penicillum expansum inoculated into sour cherry juice, apricot and peach nectars were determined based on germination tube elongation, spore germination and light and scanning electron microscopy (SEM) observations in this study. After inoculation of rate, juice/nectar samples with P. expansum spores at the level of 10(5)-10(6) cfu/mL, the samples were processed by bench scale PEF pulse generator as a function of differing electric field strengths (0, 13, 17, 20, 23, 27, 30 and 34 kV/cm) and processing times (0, 62, 94, 123, 163, 198 and 218 mu s). Results revealed that with an increase in electric field strength and processing time, germination tube elongation and spore germination rate were completely inhibited. Light and SEM observations revealed considerable morphological alterations in fungal conidia such as cytoplasmic coagulation, vacuolations, shrinkage and protoplast leakage. PEF processing of juice/nectars was demonstrated to be effective in inactivating P. expansurn. To our knowledge, this is the first study confirming the inhibitory effects of PEF on germination tube elongation and spore germination rate of P. expansum in fruit juice/nectars. (C) 2008 Elsevier Ltd. All rights reserved.
Açıklama
Anahtar Kelimeler
Pulsed Electric Fields (PEF), Sour Cherry Juice, Apricot Nectar, Peach Nectar, Penicillum Expansum, Spore Germination, Germ Tube Elongation
Kaynak
Food Microbiology
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
25
Sayı
5
