Megaporous poly(hydroxy ethylmethacrylate) based poly(glycidylmethacrylate-N-methacryloly-(L)-tryptophan) embedded composite cryogel

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dc.authorid0000-0003-0161-172Xen_US
dc.authorid0000-0002-9453-4462en_US
dc.contributor.authorTürkmen, Deniz
dc.contributor.authorBereli, Nilay
dc.contributor.authorDerazshamshir, Ali
dc.contributor.authorPerçin, Işık
dc.contributor.authorShaikh, Huma
dc.contributor.authorYılmaz, Fatma
dc.date.accessioned2021-06-23T19:42:03Z
dc.date.available2021-06-23T19:42:03Z
dc.date.issued2015
dc.departmentBAİBÜ, Gerede Meslek Yüksekokulu, Kimya Ve Kimyasal İşleme Teknolojileri Bölümüen_US
dc.description.abstractOne-step activation, purification, and stabilization of lipase enzyme were performed by using composite hydrophobic support at low ionic strength with increased surface area during embedding process. A novel hydrophobic poly(hydroxyethylmethacrylate) [PHEMA] based, poly(glycidyl methacrylate-N-methacryloly-(L)-tryptophan) [PGMATrp] bead embedded composite cryogel membrane having specific surface area of 195 m(2)/g was used as hydrophobic matrix for adsorption of commercial Candida Rugosa lipase in a continuous system. PGMATrp embedded PHEMA cryogel membrane with 60-100 mu m pore size was obtained by dispersion polymerization of GMA and MATrp to form PGMATrp beads followed by embedding of PGMATrp to HEMA via APS and TEMED redox pair. The introduction of hydrophobic MATrp monomer into bead structure aiming to increase interaction between lipase and composite membrane was estimated using nitrogen stoichiometry of elemental analysis and found to be 239 mu mol/g of polymer. Hydophobicity increment due to embedding process was confirmed by measuring contact angle, it was found 42 degrees and 48.4 degrees for the PHEMA and PHEMA/PGMATrp composite cryogel respectively. Some parameters i.e. pH, flow-rate, protein concentration, temperature, salt type and ionic intensity were evaluated on the adsorption capacity in a continuous system. Fast protein liquid chromatography (FPLC) studies were performed for specific adsorption of lipase onto the PHEMA/PGMATrp embedded composite cryogel membrane. (C) 2015 Elsevier B.V. All rights reserved.en_US
dc.identifier.doi10.1016/j.colsurfb.2015.04.004
dc.identifier.endpage68en_US
dc.identifier.issn0927-7765
dc.identifier.issn1873-4367
dc.identifier.pmid25909180en_US
dc.identifier.scopus2-s2.0-84928158355en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage61en_US
dc.identifier.urihttps://doi.org/10.1016/j.colsurfb.2015.04.004
dc.identifier.urihttps://hdl.handle.net/20.500.12491/8317
dc.identifier.volume130en_US
dc.identifier.wosWOS:000356735300009en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorYılmaz, Fatma
dc.language.isoenen_US
dc.publisherElsevier Science Bven_US
dc.relation.ispartofColloids And Surfaces B-Biointerfacesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectHICen_US
dc.subjectComposite Cryogelen_US
dc.subjectLipaseen_US
dc.subjectFPLCen_US
dc.subjectSeparationen_US
dc.titleMegaporous poly(hydroxy ethylmethacrylate) based poly(glycidylmethacrylate-N-methacryloly-(L)-tryptophan) embedded composite cryogelen_US
dc.typeArticleen_US

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