Antibody Purification from Human Plasma by Metal-Chelated Affinity Membranes

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dc.authoridArmutcu, Canan/0000-0002-0920-2843
dc.contributor.authorYavuz, Handan
dc.contributor.authorBereli, Nilay
dc.contributor.authorYilmaz, Fatma
dc.contributor.authorArmutcu, Canan
dc.contributor.authorDenizli, Adil
dc.coverage.doi10.1007/978-1-4939-2447-9
dc.date.accessioned2024-09-25T19:58:39Z
dc.date.available2024-09-25T19:58:39Z
dc.date.issued2015
dc.departmentAbant İzzet Baysal Üniversitesien_US
dc.description.abstractImmobilized metal ion affinity chromatography (IMAC) has been used for purification of proteins. IMAC introduces a new approach for selectively interacting biomolecules on the basis of their affinities for metal ions. The separation is based on different binding abilities of the proteins to the chelated metal ions on support. Here, N-methacryloyl-(L)-histidine methyl ester (MAH) is used as the metal-chelating ligand. Poly(hydroxyethyl methacrylate) Poly(HEMA) based membranes were prepared by photo-polymerization technique. Then, Zn2+, Ni2+, Co2+, and Cu2+ ions were chelated directly on the poly(HEMA-MAH) membranes for purification of immunoglobulin G (IgG) from human plasma.en_US
dc.identifier.doi10.1007/978-1-4939-2447-9_4
dc.identifier.endpage46en_US
dc.identifier.isbn978-1-4939-2447-9
dc.identifier.isbn978-1-4939-2446-2
dc.identifier.issn1064-3745
dc.identifier.pmid25749944en_US
dc.identifier.startpage43en_US
dc.identifier.urihttps://doi.org/10.1007/978-1-4939-2447-9_4
dc.identifier.urihttps://hdl.handle.net/20.500.12491/13691
dc.identifier.volume1286en_US
dc.identifier.wosWOS:000366797800005en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherHumana Press Incen_US
dc.relation.ispartofAffinity Chromatography: Methods And Protocols, 3rd Editionen_US
dc.relation.publicationcategoryKitap Bölümü - Uluslararasıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmzYK_20240925en_US
dc.subjectIMACen_US
dc.subjectAffinity membranesen_US
dc.subjectAntibody purificationen_US
dc.subjectImmunoglobulin-Gen_US
dc.subjectPoly(HEMA)en_US
dc.titleAntibody Purification from Human Plasma by Metal-Chelated Affinity Membranesen_US
dc.typeBook Chapteren_US

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