Antibody Purification from Human Plasma by Metal-Chelated Affinity Membranes
Küçük Resim Yok
Tarih
2015
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Humana Press Inc
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Immobilized metal ion affinity chromatography (IMAC) has been used for purification of proteins. IMAC introduces a new approach for selectively interacting biomolecules on the basis of their affinities for metal ions. The separation is based on different binding abilities of the proteins to the chelated metal ions on support. Here, N-methacryloyl-(L)-histidine methyl ester (MAH) is used as the metal-chelating ligand. Poly(hydroxyethyl methacrylate) Poly(HEMA) based membranes were prepared by photo-polymerization technique. Then, Zn2+, Ni2+, Co2+, and Cu2+ ions were chelated directly on the poly(HEMA-MAH) membranes for purification of immunoglobulin G (IgG) from human plasma.
Açıklama
Anahtar Kelimeler
IMAC, Affinity membranes, Antibody purification, Immunoglobulin-G, Poly(HEMA)
Kaynak
Affinity Chromatography: Methods And Protocols, 3rd Edition
WoS Q Değeri
N/A
Scopus Q Değeri
Cilt
1286
