Utilization of sucrose during cocultivation positively affects Agrobacterium-mediated transformation efficiency in sugar beet (Beta Vulgaris L.)

dc.authorid0000-0003-2379-5363
dc.authorid0000-0002-2164-5639
dc.authorid0000-0001-6201-8095
dc.authorid0000-0002-5169-8934
dc.authorid0000-0002-4421-1358
dc.authorid0000-0001-6262-2866
dc.authorid0000-0003-0070-0886
dc.contributor.authorGürel, Songül
dc.contributor.authorOğuz, Muhammet Çağrı
dc.contributor.authorTuran, Ferzat
dc.contributor.authorKazan, Kemal
dc.contributor.authorÖzmen, Canan Yüksel
dc.contributor.authorGürel, Ekrem
dc.contributor.authorErgül, Ali
dc.date.accessioned2021-06-23T18:40:06Z
dc.date.available2021-06-23T18:40:06Z
dc.date.issued2019
dc.departmentBAİBÜ, Fen Edebiyat Fakültesi, Biyoloji Bölümüen_US
dc.description.abstractSugar beet (Beta vulgaris L.) is one of the most important industrial crops throughout world. With the availability of suitable genetic transformation technologies, the yield, quality, and stress tolerance of sugar beet could be improved significantly. However, low transformation efficiencies seriously limit the application of molecular technologies to the genetic improvement of sugar beet. With the aim of improving gene transfer techniques for sugar beet, the effect of different sucrose concentrations during cocultivation on the initial Agrobacterium-mediated transformation efficiencies in sugar beet was tested. To develop an efficient experimental system through which the effect of sucrose could be tested, first, a prolific regeneration system was optimized by testing the effect of different plant growth regulators on in vitro regeneration and rooting efficiencies from sugar beet cotyledonary node explants. The highest mean number of regenerated shoots per explant was obtained when the cotyledonary node explants excised from young seedlings were grown on MS medium supplemented with 1.0 mg/L 6-benzylaminopurine. Using this regeneration system, the effect of different concentrations of sucrose included in the cocultivation medium on the initial genetic transformation efficiencies observed in T0 plants was tested using an Agrobacterium tumefaciens strain carrying the pBin19/35S:GUS-INT construct. The inclusion of 4.5% sucrose in the cocultivation medium resulted in significantly higher transformation (34.09%) and expression efficiencies (22.72%), confirmed by polymerase chain reaction and β-glucuronidase assays, respectively, in regenerated T0 seedlings. If translated into stably inherited transformation efficiencies, these findings could contribute to the success of genetic transformation studies in sugar beet and other crops recalcitrant to Agrobacterium-mediated transformation.en_US
dc.identifier.doi10.3906/tar-1812-90
dc.identifier.endpage517en_US
dc.identifier.issn1300-011X
dc.identifier.issn1303-6173
dc.identifier.issue6en_US
dc.identifier.scopus2-s2.0-85076202204en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage509en_US
dc.identifier.trdizinid335781en_US
dc.identifier.urihttps://doi.org/10.3906/tar-1812-90
dc.identifier.urihttps://app.trdizin.gov.tr/makale/TXpNMU56Z3hNUT09
dc.identifier.urihttps://hdl.handle.net/20.500.12491/2966
dc.identifier.volume43en_US
dc.identifier.wosWOS:000502956000001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.institutionauthorGürel, Songül
dc.institutionauthorGürel, Ekrem
dc.language.isoenen_US
dc.relation.ispartofTurkish Journal of Agriculture and Forestryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectSugar Beeten_US
dc.subjectGenetic Transformation
dc.subjectCocultivation
dc.subjectPlant Growth Regulators
dc.titleUtilization of sucrose during cocultivation positively affects Agrobacterium-mediated transformation efficiency in sugar beet (Beta Vulgaris L.)en_US
dc.typeArticleen_US

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