Some properties of C-phycocyanin from a native strain Anabaena affinis isolated from Kovada Lake, Isparta, Turkey

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dc.authorid0000-0001-9209-8858en_US
dc.authorid0000-0001-9209-8858en_US
dc.contributor.authorAkoğlu, Aylin
dc.contributor.authorEngin, Doruk
dc.contributor.authorÇakmakçı, M. Lütfü
dc.date.accessioned2021-06-23T19:42:11Z
dc.date.available2021-06-23T19:42:11Z
dc.date.issued2015
dc.departmentBAİBÜ, Turizm Fakültesi, Gastronomi ve Mutfak Sanatları Bölümüen_US
dc.description.abstractSome properties of C-Phycocyanin (C-PC) from a native strain Anabaena affinis isolated from Kovada Lake, Isparta, Turkey were discussed in the present. Purification process was resulted in analytical grade product with 6.93-fold increase in phycocyanin purity. By using UV-VIS and fluorescence spectrophotometry, the highest absorbance and fluorescence were determined as 620 and 646 nm, respectively. SDS-PAGE analysis revealed the presence of two major bands with a molecular mass of 17.1 and 18.6 kDa corresponding to the a and 13 subunits and as confirmed by MALDI-TOF mass spectrometric analysis. Molecular mass of pure C-PC was found as 156.2 and 198 kDa by HPLC gel filtration. Aggregation states of C-PC were estimated as hexameric-(alpha beta)(6) and trimeric forms-(alpha beta)(3) along with two binding polypeptides of 27 kDa. High antioxidant activity was determined at 480 mu M TEAC/mg phycocyanin while no antimicrobial activity was detected.en_US
dc.identifier.endpage545en_US
dc.identifier.issn0379-5136
dc.identifier.issn0975-1033
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-84978926937en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage539en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12491/8384
dc.identifier.urihttp://nopr.niscair.res.in/bitstream/123456789/34734/1/IJMS%2044%284%29%20539-545.pdf
dc.identifier.volume44en_US
dc.identifier.wosWOS:000378978200013en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorAkoğlu, Aylin
dc.language.isoenen_US
dc.publisherNatl Inst Science Communication-Niscairen_US
dc.relation.ispartofIndian Journal Of Geo-Marine Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectPhycocyaninen_US
dc.subjectCyanobacteriaen_US
dc.subjectAnabaena Affinisen_US
dc.subjectPurificationen_US
dc.subjectAntioxidant Activityen_US
dc.titleSome properties of C-phycocyanin from a native strain Anabaena affinis isolated from Kovada Lake, Isparta, Turkeyen_US
dc.typeArticleen_US

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