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Öğe Analysis of Antioxidant Effects of Lycopene and Caffeine in Experimentally Induced Renal Ischemia-Reperfusion Injury in Rats(2021) Saylan, Aslihan; Firat, Tülin; Yis, Özgür Mehmet; Dede, Gizem; Carver, HandeBackground: The aim was to investigate the antioxidant effects of lycopene and caffeine in rats exposed to renal ischemia/reperfusion (IR) injury. Materials and Methods: Following right renal nephrectomy, 60 minutes of ischemia followed by 60 minutes of reperfusion were performed in 200-250 gram Wistar albino rats used in the study. 30 minutes before ischemia, lycopene (5 mg/kg), caffeine (50 mg/kg), lycopene (5 mg/kg) + caffeine (50 mg/kg) were adminis- tered intraperitoneally to the rats. After the experiment, 4 ml of blood and left kidney were taken from the rats. Tissue malondialdehyde (MDA), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and se- rum creatinine activities were measured biochemically and light microscopy findings were evaluated histo- logically. Results: When MDA levels are compared; Although the MDA level of the IR+Lycopene group was lower than the IR group, no significance was observed. When IR+Lycopene and IR+Caffeine groups are compared; The MDA level of the IR+Lycopene group is higher than the IR+ Caffeine group. In the IR+Caffeine group; A sta- tistically significant increase was observed in serum creatinine compared to the IR+Lycopene+Caffeine group. Although SOD and GSH-Px were higher in the antioxidant groups compared to the IR group, no sta- tistically significant difference was observed. When compared with ischemia-reperfusion group, histopatho- logical damage was found to be significantly lower than in the other groups. It was found that Lycopene and Caffeine reduced apoptosis, tubular dilatation, tubular epithelium degeneration, glomerular shrinkage, des- quame epithelium and proteinous cast amount significantly. Conclusions: While lycopene has high antioxidant efficiency when compared with caffeine, lycopene and caffeine have protective effect against renal ischemia reperfusion injury.Öğe Analytical evaluation of vitamin d measurements in routine laboratories(Clin Lab Publ, 2019) Yis, Özgür Mehmet; Buğdaycı, Güler; Sönmez, Çiğdem A.; Yıldız, Rabia N.Background: Its increasing clinical importance has turned 25-hydroxy-Vitamin D (25(OH)D) into an indispensable test in clinical laboratories. In this study, we aimed to analyze the analytical performances of two widely used immunoassays, namely new restandardized Abbott product Architect 25-OH Vitamin D test and the Beckman Coulter product Access 25(OH) Vitamin D Total Test by making comparisons with the reference method liquid chromatography tandem mass spectrometry (LC-MS/MS). Methods: The new restandardized Architect I2000SR System (Abbott Laboratories, IL, USA; ref 5P02) and Access2 (Beckman Coulter, Brea, CA, USA; ref B24838) immunoassay were compared with LC-MS/MS (Shimadzu LCMS-8030, Kyoto, Japan) method 25(OH)D test for precision and reproducibility in 90 serum samples. In comparison with reference method, Deming Regression analysis and Bland Altman graphs were used. Results: Within run coefficient of variation (CV%) for Architect was found to be lower than 3.1%. Within run coefficient of variation (CV%) for Access2 was lower than 7.04%. When compared with LC-MS/MS, R value of Architect 25-OH Vitamin D kit was 0.911 (intercept 1.62, slope 1.06), mean bias was -0.04% and for Access 25(OH) Vitamin D Total kit, R value was 0.841 (intercept 9.43, slope 0.92) and mean bias of 6.9%. Conclusions: When renewed 5P02 Abbott Architect 25(OH)D and Beckman Coulter Access2 25(OH)D Total tests were compared with LC-MS/MS method in our study, they were found to have appropriate analytical values.Öğe Analytical performance evaluation of siemens sysmex CS-2500 and sekisui CP-3000(Clin Lab Publ, 2022) Pehlivan, Mine Büşra; Pehlivan, Murat; Ceylan, Celal; Alışık, Murat; Yis, Özgür Mehmet; Buğdaycı, GülerBackground: Automatic coagulation analyzers have been used in the last 50 years and have been developed considerably. Newly developed tests and methods cannot be conducted in routine laboratories without evaluating their performance. Therefore, their performance must be evaluated and approved before being used routinely. The aim of this study is to evaluate the analytical performance of Sysmex Coagulation System-2500 (CS-2500) and Sekisui CP- 3000 automatic coagulation analyzer (CP-3000). Methods: For APTT, PT, and D-dimer tests, reference range verification study, a method comparison study was performed in both analyzers in accordance with CLSI protocols, and precision and accuracy were evaluated using internal and external quality control samples. In the evaluation of precision and accuracy, CV% and bias% values were calculated. Bland-Altman, Passing-Bablok regression analysis, and correlation coefficient were used in the comparison study. Results: The CV% values calculated for APTT and PT in both analyzers were found to be below the CLSI recommendation of 5%. D-dimer test results meet the quality criteria recommended by CLSI. Accuracy for both analyzers was within the acceptable limits. The reference ranges recommended by the manufacturer have been veryfied. Regression equations for APTT, PT, and D-dimer are y = -3.313 + 1.188x, y = -0.0399 + 1.048x, and y = 0.155 + 0.655x, respectively, and r values were 0.904, 0.978, and 0.974, respectively. Conclusions: CS-2500 and CP-3000 analyzers are suitable for laboratory use for routine coagulation. Since the CP-3000 device is newly used in our country, it needs to be supported by more comparison studies.Öğe Analytical performance evaluation of siemens sysmex CS-2500 and sekisui CP-3000 (vol 68, 2022)(Clin Lab Publ, 2022) Pehlivan, Mine Büşra; Pehlivan, Murat; Ceylan, Celal; Alışık, Murat; Yis, Özgür Mehmet; Buğdaycı, GülerBackground: Automatic coagulation analyzers have been used in the last 50 years and have been developed considerably. Newly developed tests and methods cannot be conducted in routine laboratories without evaluating their performance. Therefore, their performance must be evaluated and approved before being used routinely. The aim of this study is to evaluate the analytical performance of Sysmex Coagulation System-2500 (CS-2500) and Sekisui CP- 3000 automatic coagulation analyzer (CP-3000). Methods: For APTT, PT, and D-dimer tests, reference range verification study, a method comparison study was performed in both analyzers in accordance with CLSI protocols, and precision and accuracy were evaluated using internal and external quality control samples. In the evaluation of precision and accuracy, CV% and bias% values were calculated. Bland-Altman, Passing-Bablok regression analysis, and correlation coefficient were used in the comparison study. Results: The CV% values calculated for APTT and PT in both analyzers were found to be below the CLSI recommendation of 5%. D-dimer test results meet the quality criteria recommended by CLSI. Accuracy for both analyzers was within the acceptable limits. The reference ranges recommended by the manufacturer have been veryfied. Regression equations for APTT, PT, and D-dimer are y = -3.313 + 1.188x, y = -0.0399 + 1.048x, and y = 0.155 + 0.655x, respectively, and r values were 0.904, 0.978, and 0.974, respectively. Conclusions: CS-2500 and CP-3000 analyzers are suitable for laboratory use for routine coagulation. Since the CP-3000 device is newly used in our country, it needs to be supported by more comparison studies.Öğe Analytical performance evaluation of Sysmex CS-2500 and Stago STA Compact(Lippincott Williams & Wilkins, 2020) Yis, Özgür Mehmet; Buğdaycı, Güler; Pehlivan, Mine Büşra; Yıldız, Rabia Nur; Alışık, MuratThe Sysmex Coagulation System-2500 (CS-2500) is a fully automated coagulation analyzer that uses the optical reaction method. In this study, we aimed to evaluate performance characteristics of the CS-2500 in two coagulation tests [prothrombin time (PT) and activated partial thromboplastin time (aPTT)] at our hospital laboratory. PT and aPTT measurements were performed using the CS-2500 and STA-Compact Diagnostica Stago coagulometers (STA-Compact). Then, precision, accuracy, reference range verification, and method comparison statistics were performed. In the precision study, which was performed with normal and pathologic controls for the PT-international normalized ratio (INR) and aPTT tests, all coefficient of variation% were found less than 3.5%. In the comparison study, the Passing-Bablok regression analysis demonstrated the good agreement between each analyzer for PT-INR (y = -0.081 + 1.07xandr = 0.962) and for aPTT (y = 5.498 + 0.86xandr = 0.944). Both analyzers exhibited less than 9.9% bias in the accuracy study. The reference range verification analyses revealed that the manufacturer ranges were acceptable. The verification studies of the CS-2500 coagulation system were acceptable; however, in the comparison studies, there were small differences between STA-Compact. Overall, we propose that these differences could be eliminated in future standardization studies performed to use the same reference ranges for all systems.Öğe C-reactive protein to albumin ratio in patients with prediabetes and diabetes mellitus: HbA1c and inflammation(Clinical Laboratory Publication, 2022) Demirkol, Muhammed Emin; Alışık, Murat; Yis, Özgür MehmetBackground: Inflammation has a significant status in both the pathogenesis and complications of diabetes mellitus (DM). The aim of this study is to compare the C-reactive protein (CRP) to albumin ratio (CAR) values in controlled DM, uncontrolled DM, prediabetes groups grouped by HbA1c as well as in a group of healthy individuals. Methods: In this retrospective study, 6,993 DM patients, 770 prediabetes patients, and 1,340 healthy individuals were included. According to their HbA1c levels, DM patients were divided into two groups as controlled DM (HbAlc < 6.5%, n = 4,115) and uncontrolled DM (HbAlc ??? 6.5%, n = 2,878). Results: The CRP and CAR levels were significantly higher in the DM and prediabetes group than in the control group (p < 0.05, for both). Albumin levels were significantly lower in the DM group than in both the prediabetes and control groups (p < 0.05, for both). In the uncontrolled DM group, CRP and CAR values were found to be significantly higher than the control and controlled DM groups, while albumin values were significantly lower than the control group, prediabetes group, and controlled DM group (p < 0.05, for all). Conclusions: It is thought that CAR, a liver related inflammatory marker, can be applied as an inflammation marker in both prediabetes, determined by HbA1c, and patients diagnosed with DM. Further prospective studies will better demonstrate the utility of CAR values as an inflammatory marker in DM and prediabetes.Öğe Carvacrol reduces the severity of intestinal mucosal damage caused by intestinal ischemia - Reperfusion in rats(Soc Stiinte Farmaceutice Romania, 2019) Öztürk, Hülya; Çetinkaya, Ayhan; Düzcü, Selma Erdoğan; Yis, Özgür Mehmet; Öztürk, HayrettinIntestinal ischemia-reperfusion (I/R) is a pathophysiological process that is common in many clinical conditions such as shock, sepsis, mesenteric thrombosis, necrotizing enterocolitis, and bowel transplantation. Our aim in this study was to investigate the potential protective effects of carvacrol on the intestinal I/R injury in a rat model. The 30 rats were randomly divided into three groups (n = 10): The sham-control (group 1) underwent only the separation of the superior mesenteric artery but not the occlusion. In the FR-untreated (group 2) and I/R-carvacrol-treated groups (group 3), the superior mesenteric artery was clamped for 45 min, followed by 60 min of reperfusion. 2 hours before ischemia, the group 3 of rats received an intraperitoneal injection of carvacrol at a dose of 75 mg/kg bw. At the end of the experiment, intestinal tissue samples were taken for oxidative stress assessment including superoxide dismutase (SOD), malondialdehyde (MDA), myeloperoxidase (MPO), total antioxidant status (TAS) and total oxidant status (TOS). In addition, the intestine sections were stained with haematoxylin-eosin to evaluate morphological changes and immunohistochemical staining was performed for inducible nitric oxide synthase (iNOS) and endothelial nitric oxide synthase (eNOS) assessment. The intestinal mucosa was significantly damaged in the group 2, which was markedly attenuated after carvacrol treatment. The tissue MDA, MPO and TOS content increased significantly in the group 2, but they were reduced by carvacrol treatment. In addition, SOD and TAS activity increased markedly in group 3 as compared to group 2. Immunohistochemical staining showed that iNOS increased and eNOS decreased in group 2, which was improved in reverse direction after carvacrol treatment. Carvacrol may be a potential therapeutic agent for the treatment of intestinal I/R injury.Öğe Comparison of fully automatic analyzer and manual measurement methods in sperm analysis and clinical affect(2020) Yis, Özgür MehmetAim: To investigate the clinical effect of the computer-aided sperm analyzers (CASA) by comparing the low sperm concentration semen samples evaluated by CASA with the sperm count performed on Makler Counting Chamber (MC) as a manual method.Methods: Semen samples were taken from184 patients coming to our clinic were evaluated with CASA (SQA-V Gold sperm analyzer, MES Medical Electronic Systems Ltd. Caesarea Industrial Park, IL 3088900, UK) and MC (Makler Counting Chamber, Sefi-Medical Instruments ltd., Haifa, Israel). Samples were divided into two groups as samples containing sperms and samples without sperms, according to the CASA results.Results: There was a very high correlation between the two measurement methods (rho = 0.982) and regression analysis formula was y=1.042x-0.104. No sperm was detected in CASA in any of the samples identified to have no sperm in MC. However, when patients who were identified with no sperm in their CASA measurements (n=51) were analyzed with MC, 29 patient samples (56.9%) had an average of 0.23±0.35 x106 /mL sperm.Conclusion: CASA’s used in routine semen analysis provide a great convenience in measuring sperm count, compared to manual methods and provide highly correlated results. Manual verification of samples can be recommended since the samples diagnosed with azoospermia provided different results with a manual method in our study.Öğe The effect of aromatherapy on blood pressure and stress responses by inhalation and foot massage in patients with essential hypertension randomized clinical trial(Lippincott Williams & Wilkins, 2022) Ciçek, Saadet Can; Erbaş, Şeyma Demir; Yılmaz, Dilek; Açıkgöz, Aynur; Yıldız, Sedat; Yis, Özgür MehmetA 3-arm, parallel group, randomized clinical trial examines the effect of aromatherapy through inhalation and foot massage on blood pressure and stress response in patients with essential hypertension. Lavender oil reduced blood pressure, heart rate, serum cortisol, and subjective anxiety in hypertensive patients.Öğe The effect of early tranexamic acid administration on hemoglobin levels after unstablepelvic fracture: An experimental study in rats(2021) Kaya, Yasin Emre; Arikan, Emre; Özturan, Kutay Engin; Değirmenci, Erdem; Ayanoğlu, Tacettin; Yis, Özgür MehmetAim: To investigate the effect of early systemic tranexamic acid (TRA) administration on hemoglobin (Hb)levels in rats with pelvic fracture. Method: In our study, 30 Wistar Albino rats were randomly divided into 3 groups in equal numbers and theirhemoglobin levels were measured by taking blood samples from each. No trauma was applied to the first groupand it was taken as the main control group of the study. Closed bilateral unstable pelvic fractures were createdin all rats in groups 2 and 3. Fracture creation time is considered as minute 0. 10 minutes after the fracture was formed, TRA was given to the 1st group, TRA to the 2nd group and saline solution to the 3rd groupsystemically. Hemoglobin levels were measured by taking blood samples from all rats at 30th minute and 24thhour. The initial Hb values obtained were normalized to 100 and the percentages of 30th minute and 24th hourvalues were calculated. The initial, 30th minute and 24th hour values of all groups were compared statisticallywith each other. The 30th minute and 24th hour values were compared statistically between the groups. Results: No death was observed within 24 hours in all three groups. When the first Hb values of each groupare normalized to 100, the mean Hb percentages were calculated in the first group as 99.54 and 99.84 at 30minutes and 24 hour, respectively; 92.95 and 87.73 in the second group; and 87.95 and 73.16 in the third group.When these values obtained were compared statistically within the groups (initial, 30th minute, 24th hour Hb percentages), there was no significant difference between the initial, 30th minute and 24th hour values in group1. However, a statistically significant difference was found between the initial, 30th minute and 24th hourvalues in group 2 and 3 (p<0.01). In the comparison between the groups, a statistically significant differencewas found between group 1-2, group 1-3 and group 2-3 between both 30th minute and 24th hour values(p<0.01). Conclusion: In rats with bilateral unstable pelvic fractures due to blunt pelvis trauma, early administration ofTRA after trauma significantly reduced the first 24-hour decrease in Hb value. Our study supports the earlyand prehospital use of TRA in traumas that are predicted to progress with acute bleeding, such as unstablepelvic fractures.Öğe The Effect of Wet Cupping Therapy on Anxiety and Thiol/Disulfide Homeostasis in Premature Ovarian Failure: An Experimental Study(2022) Çelik, Humeyra; Çetinkaya, Ayhan; Bozat, Bihter Gökçe; Düzcü, Selma Erdoğan; Alişik, Murat; Yis, Özgür MehmetObjective: The effect of wet cupping therapy on anxiety\rand thiol/disulfide homeostasis was evaluated in rats with premature\rovarian failure (POF) induced by cyclophosphamide (CYC). Material\rand Methods: Twenty four Wistar Albino female rats were divided\rinto control (n=6), POF (n=6), wet cupping (n=6), POF+wet cupping\r(n=6) groups. CYC at a dose of 200 mg/kg was injected intraperitoneally\rto POF and POF+wet cupping groups. One week later, wet cupping\rtherapy was applied to wet cupping and POF+wet cupping groups for\r5 minutes. The next day, Open Field Test and Elevated Plus Maze Test\rwere applied to all experimental groups to evaluate anxiety. After sacrification,\rto assess pattern formation, endometrial thickness and ovarian\rreserve were measured with hematoxylin-eosin staining.\rThiol/disulfide homeostasis and erythrocyte oxidized-reduced glutathione\rlevels were measured in serum to measure oxidative stress. Results:\rAlthough there was no statistical significance, it was observed\rthat anxiety decreased in POF+wet cupping compared to POF group.\rThere was no change in thiol-disulfide values in POF+wet cupping\rcompared to POF group (p>0.05). Conclusion: Wet cupping therapy\rdid not reduce anxiety in the experimental POF model and did not shift\rthe balance to positive in thiol/disulfide homeostasis in favor of thiols.\rHowever, the obtained graphics, numerical data and high standard deviation\rsuggest that meaningful data could be obtained with more subjects.Öğe Effects of photobiomodulation therapy on human sperm function(Elsevier España S.L.U., 2023) Saylan, Aslıhan; Fırat, Tülin; Yis, Özgür MehmetIntroduction: Sperm motility is a crucial factor in male infertility and it depends on mitochondrial tail movements. Photobiomodulation light therapy allows the cells to produce their energy through activation of the mitochondria. The aim of the present study was to examine the impact of photobiomodulation on sperm motility in astenozoospermic individuals. Materials and methods: Following semen analyses of 20 astenozoospermic individuals, collected semen samples were centrifuged. Pellet was obtained and homogenized through mixing with culture media in 1:1 ratio. Each semen samples were divided into 3 groups. In the first group, control samples were not exposed to laser irradiation. The Group 2 and Group 3 were exposed to 650 nm wavelength of photobiomodulation from 10 cm distance in dark environment via a 36 cm(2) aperture sizer with 200 mW output power for 30 and 60 min duration, respectively. Sperm motilities were evaluated and chromatin condensation of sperms was determined. Results: Sperm motilities were significantly increased in photobiomodulation groups compared with the controls. Sperm motilities tended to be different between the 30 and 60 min red light exposure groups; however, it was not statistically significant. When the motility grades were compared, no significant difference was observed in non-progressive motility sperms. While immotile sperms decreased significantly in the photobiomodulation groups compared to the control group, progressive sperms increased. Conclusions: The results of the present study demonstrated that the photobiomodulation is an efficient method to increase the sperm motility of astenozoospermic individuals independent of the duration of exposure. (c) 2022 Asociaci ' on Espanola de Androlog ' ia, Medicina Sexual y Reproductiva. Published by Elsevier Espana, S.L.U. All rights reserved.Öğe Evaluation of Bio-Rad D-10® and arkray adams HA-8160® HPLC analyzers in HbA1c measurement(2019) Yis, Özgür Mehmet; Pehlivan, Mine Büşra; Buğdaycı, Güler; Yüce, NeslihanGlycosylated hemoglobin A1c (HbA1c) is an important parameter used for the assessment of time-dependent glycemic status and the diagnosis and follow-up of diabetes. This study aimed to evaluate the analytical performance of two HPLC analyzers, the Bio-Rad D-10®, and the Arkray Adams HA-8160®. Accuracy and imprecision studies were conducted, and a method comparison study was performed with 105 samples. Samples were collected on five consecutive days and measured on both analyzers within two hours. Bland-Altman and regression analyses were used for statistical evaluation of the data. Low and high-level within-day CV values were calculated as 1.22% and 0.60% for the Arkray analyzer and 1.2% and 0.30% for the Bio-Rad analyzer, respectively. They were calculated as 1.27% and 2.52% for the Arkray analyzer and 2.32% and 3.44% for the Bio-Rad analyzer, respectively, between days. The within-day CV values for both analyzers were below the limit of 2.5% specified by the International Federation of Clinical Chemistry (IFCC). The bias average for three months was 3.2% for the Arkray analyzer and 1.2% for the Bio-Rad analyzer. The Spearman correlation coefficient (r) was 0.973 (p<0.0001) between the two measurements, and the slope and intercept values calculated in the regression analyses showed the linear relationship and harmony between the two analyzers [R2 = 0.967, (y = 0.860x + 0.604)]. Both HPLC analyzers are shown to be reproducible and accurate for routine biochemistry laboratory use.Öğe Evaluation of the analytical performance of lifotronic H9 glycated hemoglobin analyzer and its effect on clinical decision making(Clin Lab Publ, 2020) Yis, Özgür MehmetBackground: We aimed to analyze the analytical performance of Lifotronic H9 glycated hemoglobin analyzer (Li-fotronic H9) with Arkray Adams HA-8160 glycated hemoglobin analyzer (Arkray HA8160), which is currently used for measurement of HbA1c test in our laboratory. Methods: The verification studies of the Lifotronic H9 were performed by comparison with the Arkray HA8160, which has been verified and is already used in our laboratory, and in accordance with the CLSI EP5-A3 and CLSI 15-A3 guidelines. The method comparison study was performed with 316 subjects. Results: Both instruments demonstrated perfect repeatability (within-run) CVs (0.8% - 1.6%), between-run CVs (0.2% - 1.0%), within-day CVs (0.8% - 1.7%), between-day CVs (0.3% - 0.6%), and within laboratory (total imprecision) CVs (0.8% - 1.8%) with two levels of quality control materials. Very good linearity was observed in the method-comparison studies based on Passing-Bablok regression analysis (y = -0.03 + 1.02 x, CUSUM test p = 0.07). Intraclass correlation coefficient (ICC = 0.995) for results and Kappa value (kappa = 0.86) for classification of results according to the American Diabetes Association recommendations showed almost perfect agreement. Conclusions: The Lifotronic H9 analyzer shows good precision with lower than recommended total CV 2% and linearity with Arkray HA8160. The Lifotronic H9 is a suitable analyzer for the diagnosis of diabetes and monitoring diabetic control.Öğe Evaluation of the analytical performance of two vitamin D immunoassay methods(2019) Yis, Özgür Mehmet; Buğdaycı, Güler; Yıldız, Rabia Nur; Oğuzman, HamdiObjectives: The increasing clinical significance of 25-hydroxyvitamin D [25(OH)D] has made it an indispensable test in the laboratory. The aim of this study was to investigate the analytical performance of 2 widely used immunoassay methods, the new Abbott Architect 5P02 25-OH Vitamin D assay (Abbott Laboratories, Lake Bluff, IL, USA) and the Beckman Coulter product, the Access 25 (OH) Vitamin D Total assay (Beckman Coulter, Inc., Brea, CA, USA). Methods: A total of 96 serum samples were used to compare the accuracy, linearity, limit of blank, limit of detection, limit of quantification, and precision of the 2 tests using the Architect i2000SR System (Abbott Laboratories, Lake Bluff, IL, USA) and the Access2 (Beckman Coulter, Brea, CA, USA) immunoassay platform. Deming regression analysis and Bland Altman graphs were used to compare the 2 methods. Results: The within-run coefficient of variation (CV%) of the Architect and the Access systems was <3.1% and <6.72%, respectively. There was good linearity of the 25(OH)D measurement at 98.9-114% for the Architect assay and 94.1- 103.5% for the Access assay at concentrations of 5-160 ng/mL and 6.2-210.4 ng/mL, respectively. The correlation coefficient was 0.921 (y=-2.1+0.97x). Conclusion: Thorough testing indicated that the new 5P02 Abbott Architect 25(OH)D and the Beckman Coulter Access2 25(OH)D Total test demonstrated appropriate analytical performance.Öğe Expression of NGF, MCP-1, uroplakin III, and NOS in bladder urothelium after partial urethral obstruction in rats(Elsevier Sci Ltd, 2020) Öztürk, Hayrettin; Çetinkaya, Ayhan; Düzcü, Selma Erdoğan; Yis, Özgür MehmetIntroduction Although several cytokines, chemokines, and growth factors have been suggested to play a role in the development of bladder fibrosis and functional changes, the mechanisms that are effective in the pathogenesis of partial bladder outlet obstruction (pB00)-induced bladder fibrosis are not well understood. Objective We investigated the expressions of nerve growth factor (NGF), monocyte chemoattractant protein-1 (MCP-1), uroplakin III (URPIII), inducible nitric oxide synthase (iNOS), and endothelial NOS (eNOS) that may be involved in fibrosis in rats with partial urethral obstruction for 1, 2 and 3 weeks, and the changes in the associated ischemic and inflammatory processes. After 1, 2, and 3 weeks of pB00, blood samples were collected for assessment of renal function from the rats under anesthesia. The bladders were dissected for the tissue antioxidant enzyme activities and lipid peroxidation, including malondialdehyde (MDA), superoxide dismutase (SOD), total antioxidant status (TAS) and total oxidant status (TOS). The immunohistochemical studies were performed. Histopathologically, the number of urothelial layers was calculated and the thickness of the detrusor smooth muscle and lamina propria were quantitatively measured. Additionally, the edema and congestion in the submucosa were evaluated. Study design Twenty-eight male Sprague-Dawley rats were used in this study. Three separate experimental groups of pB00 (1 week [n = 7], 2 weeks [n = 7], and 3 weeks [n = 7]) were created, with an additional sham-operated control group (n = 7). Results Study design Twenty-eight male Sprague-Dawley rats were used in this study. Three separate experimental groups of pB00 (1 week [n = 7], 2 weeks [n = 7], and 3 weeks [n = 7]) were created, with an additional sham-operated control group (n = 7). Results The MDA levels increased in pBOO groups. The SOD values were decreased in the pBOO group for 1 week, and higher in the 3-week pBOO group. The TAS levels were increased in the 3 week pB00 group. The TOS levels increased in the pBOO groups. The number of urothelial layers was decreased in pBOO groups. The lamina propria, the smooth muscle thickness, edema and congestion were increase in the 1 and 2 week pBOO groups. The NGF and MCP-1 expression was increased in the 1-week and 2-week pBOO groups. The expression of URPIII in the epithelium gradually increased in the pBOO groups. In the pBOO groups, iNOS expression in the epithelium cells was significantly elevated. However, the eNOS expression was also significantly increased in the 2 week pBOO group. Conclusion Our study shows that overexpression of immunohistochemical parameters together with the negative effects of ischemic and inflammatory processes that subjected to pB00 for 1, 2 and 3 weeks may play a potential role in detrusor fibrosis in the rat bladders induced by pB00. However, understanding of the immunohistochemical parameters investigated in this experimental study is limited, and further studies targeting their relationship to pB00 could help us develop new strategies.Öğe Is neuregulin-4 a predictive marker of microvascular complications in type 2 diabetes mellitus?(Wiley, 2020) Koçak, Mehmet Zahid; Aktaş, Gülali; Tel, Meryem Burçin Atak; Duman, Tuba Taslamacıoğlu; Yis, Özgür Mehmet; Erkuş, Edip; Şavlı, HalukBackground and Aims Neuregulin-4 (Nrg-4) is a new adipokine released from brown adipose tissue. It plays pivotal role in regulating systemic energy balance, glucose and lipid metabolism and in reducing chronic inflammation. We aimed to investigate the relation between diabetic microvascular complications (DMC) and serum (Nrg-4) levels in patients with type 2 diabetes mellitus. Methods Patients with type 2 diabetes mellitus were divided into DMC and diabetic patients without microvascular complications (non-DMC). Nrg-4 levels of the patients were compared. Results Fifty and 29 patients enrolled to the DMC and non-DMC groups, respectively. Nrg-4 was 1.23 (0.02-5.1) ng/mL and 2.5 (0.21-6.01) ng/mL in DMC and in non-DMC groups, respectively (P < .001). In patients with DMC, FPG was 189.5 (89-446) mg/ dL, whereas it was 128 (95-278) mg/dL in non-DMC diabetic patients (P < .001). HbA1c was also significantly higher in the DMC group than in the non-DMC group (P < .001). Negative correlation was found between Nrg-4 and FPG (r = -0.231, P = .03), HBA1c (r = -0.312, P = .003) and microalbuminuria (r = -0.277, P = .009). Logistic regression analysis showed a 1-unit decrease in Nrg-4 to increase the presence of DMC by 1.9 times. The best cut-off value of Nrg-4 was 1.56 ng/mL with 82.1% sensitivity and 64% specificity, in predicting DMC. Conclusion In patients with diabetes, Nrg-4 levels may be a good predictor of early detection of one or more DMC, as microvascular dysfunction in an organ system is considered to be an initial onset of subclinical systemic damage.Öğe Is the prognostic nutritional index a predictor of Covid-19 related hospitalizations and mortality?(Kamuzu Univ Health Sciences - KUHeS, 2023) Demirkol, Muhammed Emin; Aktaş, Gülali; Alışık, Murat; Yis, Özgür Mehmet; Kaya, Musa; Kocadağ, DeryaIntroduction Prognostic nutritional index (PNI) is a novel inflammation marker that useful in predicting prognosis of certain conditions. We aimed to study PNI of the outpatient and inpatient subjects with established Covid-19 and also aimed to compare PNI of deceased and survived Covid-19 patients. Methods The patients with Covid-19 whom presented to outpatient or inpatient clinics of Abant Izzet Baysal University Hospital were enrolled to the study. PNI levels of the inpatients and outpatients, deceased and survived were compared. PNI values of deceased and survived in inpatients were also compared. Results Study population was consisted of 4419 subjects (2907 outpatients and 1512 inpatients). PNI of the inpatient (41.55 (36.42-47.1)) group was significantly lower than the PNI of the outpatient (51.95 (47.95-55.75)) subjects (p<0.001). The sensitivity and specificity of PNI (<= 46.2 level) in determination of requirement inpatient treatment were 71.2% and 83.5%, respectively. PNI of the deceased patients (37(33.39-40.86)) was lower than the PNI of the survivors (50.45(45.6-54.65)), (p<0.001). The sensitivity and specificity of PNI at <= 44.55 level in determining mortality were 89.22% and 78.87%, respectively. Conclusion We suggest that PNI could serve as a reliable prognostic index in covid-19 patients. Reduced level of PNI should alert physicians since it is associated with need for hospitalization and mortality in this population.Öğe Minoxidil reduces testicular damage in experimental testicular ischemia/reperfusion injury(Innovative Scientific Information & Services Network, 2020) Öztürk, Hayrettin; Düzcü, Selma Erdoğan; Yis, Özgür Mehmet; Çetinkaya, Ayhan; Cevizci, Nuri; Öztürk, HülyaWe investigated the effects of K-ATP channel opener minoxidil on oxidant stress, antioxidant defense and histopathological damage in testicular torsion-induced rats. A total of 21 male Wistar-Albino rats were used. Group 1 (the sham-control): the left testis and spermatic cord were only released with a scrotal incision. Group 2 (I/R-untreated): the left testis and spermatic cord was rotated clockwise by 720 degrees for 60 minutes and detorsioned for 60 minutes. Group 3 (I/R-minoxidil treated): Testicular torsion was created as in the group 2 rats and 0.3 mg /kg minoxidil was injected. The testes were extracted for biochemical and histopathological examination.Minoxidil treatment showed a significant decrease in MDA and MPO levels. In the group 2, there was a decrease in TAS levels and an increase in TOS levels. Minoxidil treatment resulted in an improvement of these values in the testicular tissues. There was an improvement in the testicular injury score with minoxidil treatment. The group 2 rats had grade III and grade IV lesions. The treatment with minoxidil showed lower histopathologic features in the testicular tissues. Minoxidil may play a protective role in reducing damage caused by reperfusion of the ischemic testicular tissues.Öğe Multiple calculi in a single-system orthotopic ureterocele in a two-year-old boy presenting with hematuria: A case report(2018) Öztürk, Hülya; Bektaş, Mervan; Yis, Özgür MehmetUreterocele is the dilatation of the submucosal ureter as a result of a defect in the Chwalla membrane absorption. The many types and clinical presentations of ureters present a significant challenge to clinicians. Although stone formation in the ureterocele is common in adults, it is much less common in pediatric patients. Here, we present a case report of a two-year-old boy with single-system ureteroceles containing multiple small stones.
